Nanoparticles containing anti-inflammatory agents as chemotherapy adjuvants: Optimization and in vitro characterization

Xiuling Lu, Melissa D. Howard, Marta Mazik, Joshua Eldridge, John J. Rinehart, Michael Jay, Markos Leggas

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


The pre-administration of dexamethasone (DEX) has previously been shown to enhance the anti-tumor efficacy of chemotherapeutic agents. The delivery of anti-inflammatory agents specifically to tumors via nanoparticle carriers is expected to promote the effectiveness of chemotherapeutic agents while avoiding systemic toxicities. The process for preparing solid lipid nanoparticles containing anti-inflammatory agents using the nanotemplate engineering method was optimized. Due to the solubilization of DEX in the bulk aqueous phase, its more lipophilic palmitate ester was synthesized and incorporated in nanoparticles that included a pegylating agent, PEG6000 mono-stearate, as part of the formulation. The stealth properties of these nanoparticles were demonstrated to be enhanced compared to latex particles by measuring the adsorption of radioiodinated IgG (185 μg vs. 6.7 μg IgG/mg NP). In addition, the uptake of 14C-labeled nanoparticles by murine macrophages was shown to decrease from 36.6% to 14.7% of the nanoparticles/mg cell protein as the amount of pegylating agent in the formulation increased from 0 to 4 mg/mL. The high loading values and low burst effect observed for these DEX palmitate-containing nanoparticles in addition to their stealth properties are expected to allow for the delivery of sufficient amounts of DEX to tumors to enhance the uptake of chemotherapeutic agents.

Original languageEnglish
Pages (from-to)133-140
Number of pages8
JournalAAPS Journal
Issue number1
StatePublished - 2008

Bibliographical note

Funding Information:
The authors are grateful for financial support from the Kentucky Cancer Experimental Therapeutics Program and Benedict Cassen Postdoctoral Fellowship from the Education and Research Foundation.


  • Adsorption
  • Biodegradable
  • Dexamethasone
  • Macrophage
  • Microemulsion
  • Nanoparticle
  • Tumor

ASJC Scopus subject areas

  • Pharmaceutical Science


Dive into the research topics of 'Nanoparticles containing anti-inflammatory agents as chemotherapy adjuvants: Optimization and in vitro characterization'. Together they form a unique fingerprint.

Cite this