Nanopore sequencing of clonal IGH rearrangements in cell-free DNA as a biomarker for acute lymphoblastic leukemia

Shilpa Sampathi, Yelena Chernyavskaya, Meghan G. Haney, L. Henry Moore, Isabel A. Snyder, Anna H. Cox, Brittany L. Fuller, Tamara J. Taylor, Donglin Yan, Tom C. Badgett, Jessica S. Blackburn

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Background: Acute Lymphoblastic Leukemia (ALL) is the most common pediatric cancer, and patients with relapsed ALL have a poor prognosis. Detection of ALL blasts remaining at the end of treatment, or minimal residual disease (MRD), and spread of ALL into the central nervous system (CNS) have prognostic importance in ALL. Current methods to detect MRD and CNS disease in ALL rely on the presence of ALL blasts in patient samples. Cell-free DNA, or small fragments of DNA released by cancer cells into patient biofluids, has emerged as a robust and sensitive biomarker to assess cancer burden, although cfDNA analysis has not previously been applied to ALL. Methods: We present a simple and rapid workflow based on NanoporeMinION sequencing of PCR amplified B cell-specific rearrangement of the (IGH) locus in cfDNA from B-ALL patient samples. A cohort of 5 pediatric B-ALL patient samples was chosen for the study based on the MRD and CNS disease status. Results: Quantitation of IGH-variable sequences in cfDNA allowed us to detect clonal heterogeneity and track the response of individual B-ALL clones throughout treatment. cfDNA was detected in patient biofluids with clinical diagnoses of MRD and CNS disease, and leukemic clones could be detected even when diagnostic cell-count thresholds for MRD were not met. These data suggest that cfDNA assays may be useful in detecting the presence of ALL in the patient, even when blasts are not physically present in the biofluid sample. Conclusions: The Nanopore IGH detection workflow to monitor cell-free DNA is a simple, rapid, and inexpensive assay that may ultimately serve as a valuable complement to traditional clinical diagnostic approaches for ALL.

Original languageEnglish
Article number958673
JournalFrontiers in Oncology
Volume12
DOIs
StatePublished - Dec 14 2022

Bibliographical note

Publisher Copyright:
Copyright © 2022 Sampathi, Chernyavskaya, Haney, Moore, Snyder, Cox, Fuller, Taylor, Yan, Badgett and Blackburn.

Keywords

  • B-cell acute lymphoblastic leukemia (B-ALL)
  • VDJ rearrangement
  • central nervous system (CNS) disease
  • clonality
  • minimal residual disease (MRD)
  • relapse

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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