Nanoscale live-cell imaging using hopping probe ion conductance microscopy

Pavel Novak, Chao Li, Andrew I. Shevchuk, Ruben Stepanyan, Matthew Caldwell, Simon Hughes, Trevor G. Smart, Julia Gorelik, Victor P. Ostanin, Max J. Lab, Guy W.J. Moss, Gregory I. Frolenkov, David Klenerman, Yuri E. Korchev

Research output: Contribution to journalArticlepeer-review

430 Scopus citations

Abstract

We describe hopping mode scanning ion conductance microscopy that allows noncontact imaging of the complex three-dimensional surfaces of live cells with resolution better than 20 nm. We tested the effectiveness of this technique by imaging networks of cultured rat hippocampal neurons and mechanosensory stereocilia of mouse cochlear hair cells. The technique allowed examination of nanoscale phenomena on the surface of live cells under physiological conditions.

Original languageEnglish
Pages (from-to)279-281
Number of pages3
JournalNature Methods
Volume6
Issue number4
DOIs
StatePublished - 2009

Bibliographical note

Funding Information:
This study was supported by the UK Biotechnology and Biological Sciences Research Council and Medical Research Council to Y.E.K. and (BB/D01817X/1) to G.W.J.M. and T.G.S., and by the US National Organization for Hearing Research Foundation, the Kentucky Science and Engineering Foundation (KSEF-148-502-07-215) and National Institute on Deafness and Other Communication Disorders, National Institutes of Health (DC008861 to G.I.F.).

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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