Nanoscale live-cell imaging using hopping probe ion conductance microscopy

Pavel Novak; Chao Li; Andrew I. Shevchuk; Ruben Stepanyan; Matthew Caldwell; Simon Hughes; Trevor G. Smart; Julia Gorelik; Victor P. Ostanin; Max J. Lab; Guy W.J. Moss; Gregory I. Frolenkov; David Klenerman; Yuri E. Korchev

doi:10.1038/nmeth.1306

Nanoscale live-cell imaging using hopping probe ion conductance microscopy

Pavel Novak, Chao Li, Andrew I. Shevchuk, Ruben Stepanyan, Matthew Caldwell, Simon Hughes, Trevor G. Smart, Julia Gorelik, Victor P. Ostanin, Max J. Lab, Guy W.J. Moss, Gregory I. Frolenkov, David Klenerman, Yuri E. Korchev

Physiology

Research output: Contribution to journal › Article › peer-review

448 Scopus citations

Abstract

We describe hopping mode scanning ion conductance microscopy that allows noncontact imaging of the complex three-dimensional surfaces of live cells with resolution better than 20 nm. We tested the effectiveness of this technique by imaging networks of cultured rat hippocampal neurons and mechanosensory stereocilia of mouse cochlear hair cells. The technique allowed examination of nanoscale phenomena on the surface of live cells under physiological conditions.

Original language	English
Pages (from-to)	279-281
Number of pages	3
Journal	Nature Methods
Volume	6
Issue number	4
DOIs	https://doi.org/10.1038/nmeth.1306
State	Published - 2009

Bibliographical note

Funding Information:
This study was supported by the UK Biotechnology and Biological Sciences Research Council and Medical Research Council to Y.E.K. and (BB/D01817X/1) to G.W.J.M. and T.G.S., and by the US National Organization for Hearing Research Foundation, the Kentucky Science and Engineering Foundation (KSEF-148-502-07-215) and National Institute on Deafness and Other Communication Disorders, National Institutes of Health (DC008861 to G.I.F.).

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth.1306

Cite this

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abstract = "We describe hopping mode scanning ion conductance microscopy that allows noncontact imaging of the complex three-dimensional surfaces of live cells with resolution better than 20 nm. We tested the effectiveness of this technique by imaging networks of cultured rat hippocampal neurons and mechanosensory stereocilia of mouse cochlear hair cells. The technique allowed examination of nanoscale phenomena on the surface of live cells under physiological conditions.",

author = "Pavel Novak and Chao Li and Shevchuk, {Andrew I.} and Ruben Stepanyan and Matthew Caldwell and Simon Hughes and Smart, {Trevor G.} and Julia Gorelik and Ostanin, {Victor P.} and Lab, {Max J.} and Moss, {Guy W.J.} and Frolenkov, {Gregory I.} and David Klenerman and Korchev, {Yuri E.}",

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doi = "10.1038/nmeth.1306",

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AU - Novak, Pavel

AU - Li, Chao

AU - Shevchuk, Andrew I.

AU - Stepanyan, Ruben

AU - Caldwell, Matthew

AU - Hughes, Simon

AU - Smart, Trevor G.

AU - Gorelik, Julia

AU - Ostanin, Victor P.

AU - Lab, Max J.

AU - Moss, Guy W.J.

AU - Frolenkov, Gregory I.

AU - Klenerman, David

AU - Korchev, Yuri E.

N1 - Funding Information: This study was supported by the UK Biotechnology and Biological Sciences Research Council and Medical Research Council to Y.E.K. and (BB/D01817X/1) to G.W.J.M. and T.G.S., and by the US National Organization for Hearing Research Foundation, the Kentucky Science and Engineering Foundation (KSEF-148-502-07-215) and National Institute on Deafness and Other Communication Disorders, National Institutes of Health (DC008861 to G.I.F.).

PY - 2009

Y1 - 2009

N2 - We describe hopping mode scanning ion conductance microscopy that allows noncontact imaging of the complex three-dimensional surfaces of live cells with resolution better than 20 nm. We tested the effectiveness of this technique by imaging networks of cultured rat hippocampal neurons and mechanosensory stereocilia of mouse cochlear hair cells. The technique allowed examination of nanoscale phenomena on the surface of live cells under physiological conditions.

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Nanoscale live-cell imaging using hopping probe ion conductance microscopy

Abstract

Bibliographical note

ASJC Scopus subject areas

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