Nasal Shedding of Equid Herpesvirus Type 1 and Type 4 in Hospitalized, Febrile Horses

Jennifer M. Sonis; Lutz S. Goehring

doi:10.1016/j.jevs.2012.11.002

Nasal Shedding of Equid Herpesvirus Type 1 and Type 4 in Hospitalized, Febrile Horses

Jennifer M. Sonis, Lutz S. Goehring

Veterinary Science

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The objective of this study was to determine the prevalence of shedding of equid herpesvirus 1 (EHV-1) or EHV-4 in nasal swab samples from any febrile, hospitalized horses during a 1-year period. It was hypothesized that some fevers in horses are associated with viral replication following recrudescence of latent virus or following a horizontal viral infection prior to or during admission to a referral hospital. During the observational period, nasal swab samples were collected from 64 febrile and 10 nonfebrile hospitalized horses. Routine DNA extraction was performed, and a validated quantitative polymerase chain reaction (qPCR) assay was used to detect and quantify genomic EHV-1 and -4 DNA. Genomic DNA of EHV-4 was detected in the nasal swab specimen of 1 of 64 febrile horses. EHV-1 DNA was not detected in any of the febrile horses. Samples from all nonfebrile horses were negative for both viruses. Considering the known association between fever and shedding of EHV-1 and EHV-4, we anticipated finding a higher percentage of PCR-positive samples from febrile patients. Fevers detected were likely a result of active disease processes for which the horses were hospitalized; concurrent other diseases appeared not to affect viral recrudescence. Further studies are warranted to examine frequency and factors of EHV latency and reactivation.

Original language	English
Pages (from-to)	756-759
Number of pages	4
Journal	Journal of Equine Veterinary Science
Volume	33
Issue number	9
DOIs	https://doi.org/10.1016/j.jevs.2012.11.002
State	Published - Sep 2013

Bibliographical note

Funding Information:
The work was completed at the James L. Voss Veterinary Teaching Hospital, Colorado State University, Fort Collins, CO. The study was supported by a grant from the Animal Population Health Institute at Colorado State University . An abstract was presented at the Forum of the American College of Veterinary Internal Medicine held in Anaheim, CA, June 9-12, 2010. Our special thanks to Dr. Brandy Burgess for carefully editing the script.

Keywords

Detection
Equid herpesvirus
Fever
Latency recrudescence
Nasal swab

ASJC Scopus subject areas

Equine

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10.1016/j.jevs.2012.11.002

Cite this

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title = "Nasal Shedding of Equid Herpesvirus Type 1 and Type 4 in Hospitalized, Febrile Horses",

abstract = "The objective of this study was to determine the prevalence of shedding of equid herpesvirus 1 (EHV-1) or EHV-4 in nasal swab samples from any febrile, hospitalized horses during a 1-year period. It was hypothesized that some fevers in horses are associated with viral replication following recrudescence of latent virus or following a horizontal viral infection prior to or during admission to a referral hospital. During the observational period, nasal swab samples were collected from 64 febrile and 10 nonfebrile hospitalized horses. Routine DNA extraction was performed, and a validated quantitative polymerase chain reaction (qPCR) assay was used to detect and quantify genomic EHV-1 and -4 DNA. Genomic DNA of EHV-4 was detected in the nasal swab specimen of 1 of 64 febrile horses. EHV-1 DNA was not detected in any of the febrile horses. Samples from all nonfebrile horses were negative for both viruses. Considering the known association between fever and shedding of EHV-1 and EHV-4, we anticipated finding a higher percentage of PCR-positive samples from febrile patients. Fevers detected were likely a result of active disease processes for which the horses were hospitalized; concurrent other diseases appeared not to affect viral recrudescence. Further studies are warranted to examine frequency and factors of EHV latency and reactivation.",

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note = "Funding Information: The work was completed at the James L. Voss Veterinary Teaching Hospital, Colorado State University, Fort Collins, CO. The study was supported by a grant from the Animal Population Health Institute at Colorado State University . An abstract was presented at the Forum of the American College of Veterinary Internal Medicine held in Anaheim, CA, June 9-12, 2010. Our special thanks to Dr. Brandy Burgess for carefully editing the script.",

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Nasal Shedding of Equid Herpesvirus Type 1 and Type 4 in Hospitalized, Febrile Horses

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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