Natriuretic and kaliuretic activities of guanylin and uroguanylin in the isolated perfused rat kidney

Manasses C. Fonteles, Richard N. Greenberg, Helena S.A. Monteiro, Mark G. Currie, Leonard R. Forte

Research output: Contribution to journalArticlepeer-review

109 Scopus citations


Guanylin and uroguanylin are novel peptides that activate membrane guanylate cyclases found in the kidney and intestine. We compared the effects of these peptides in the isolated perfused rat kidney. Both peptides are natriuretic and kaliuretic in this preparation. Uroguanylin (0.19-1.9 μM) increased glomerular filtration rate from 0.77 ± 0.07 to 1.34 ± 0.3 ml·g- 1·min-1 at the highest concentration. A maximal increase in Na+ excretion was achieved at 0.66 μM uroguanylin, with a reduction in fractional Na+ reabsorption from 78.7 ± 1.7 to 58.8 ± 4.4%. The highest dose of uroguanylin increased kaliuresis by 50%. Osmolar clearance doubled at the highest concentration of uroguanylin tested (P < 0.05). Guanylin also elicited a natriuresis and kaliuresis but appeared to be less potent than uroguanylin. The highest concentration of guanylin (1.3 μM) decreased fractional Na+ reabsorption from 73.9 ± 2.4 to 64.5 ± 4.0%, but lower doses were ineffective. Guanylin stimulated urine K+ excretion at the lowest concentration tested (0.33 μM) without any effect on Na+ excretion. These peptides may influence salt and water homeostasis by biological effects in the kidney that are mediated by the intracellular second messenger, cGMP.

Original languageEnglish
Pages (from-to)F191-F197
JournalAmerican Journal of Physiology - Renal Physiology
Issue number2 44-2
StatePublished - Aug 1998


  • Glomerular filtration
  • Sodium and potassium transport

ASJC Scopus subject areas

  • Physiology
  • Urology


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