NC1 domain of human type VIII collagen (α 1) inhibits bovine aortic endothelial cell proliferation and causes cell apoptosis

Ren Xu, Zhong Yin Yao, Li Xin, Qian Zhang, Tsai Ping Li, Ren Bao Gan

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

Endostatin, a natural angiogenesis inhibitor, had been identified for years. It opened a new approach for cancer therapy. Sequence analysis reveled that endostatin is the NC1 domain (non-triple-helical domain) of collagen XVIII. In this report, the cDNA of NC1 domain of type VIII collagen (α 1) was cloned and expressed as soluble form in Escherichia coli. The recombinant protein was purified with Ni-NTA agarose column and named as vastatin. It inhibited the proliferation of bovine aortic endothelial (BAE) cell stimulated by basic fibroblast growth factor (bFGF) in a dose-dependent manner. The ED50 of vastatin was 0.6 μg/ml, while the ED50 of endostatin was 0.5 μg/ml. Treatment of BAE cell with vastatin caused G0-G1 arrest and cell apoptosis. It is interesting that sequence analysis showed that there was only about 12% amino acid sequence homology between vastatin and endostatin. The structure - function relationship of these angiogenesis molecules remains to be elucidated.

Original languageEnglish
Pages (from-to)264-268
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume289
Issue number1
DOIs
StatePublished - Nov 23 2001

Keywords

  • Angiogenesis inhibitor
  • Apoptosis
  • NC1 domain
  • Type VIII collagen
  • Vastatin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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