TY - JOUR
T1 - NC1 domain of human type VIII collagen (α 1) inhibits bovine aortic endothelial cell proliferation and causes cell apoptosis
AU - Xu, Ren
AU - Yao, Zhong Yin
AU - Xin, Li
AU - Zhang, Qian
AU - Li, Tsai Ping
AU - Gan, Ren Bao
PY - 2001/11/23
Y1 - 2001/11/23
N2 - Endostatin, a natural angiogenesis inhibitor, had been identified for years. It opened a new approach for cancer therapy. Sequence analysis reveled that endostatin is the NC1 domain (non-triple-helical domain) of collagen XVIII. In this report, the cDNA of NC1 domain of type VIII collagen (α 1) was cloned and expressed as soluble form in Escherichia coli. The recombinant protein was purified with Ni-NTA agarose column and named as vastatin. It inhibited the proliferation of bovine aortic endothelial (BAE) cell stimulated by basic fibroblast growth factor (bFGF) in a dose-dependent manner. The ED50 of vastatin was 0.6 μg/ml, while the ED50 of endostatin was 0.5 μg/ml. Treatment of BAE cell with vastatin caused G0-G1 arrest and cell apoptosis. It is interesting that sequence analysis showed that there was only about 12% amino acid sequence homology between vastatin and endostatin. The structure - function relationship of these angiogenesis molecules remains to be elucidated.
AB - Endostatin, a natural angiogenesis inhibitor, had been identified for years. It opened a new approach for cancer therapy. Sequence analysis reveled that endostatin is the NC1 domain (non-triple-helical domain) of collagen XVIII. In this report, the cDNA of NC1 domain of type VIII collagen (α 1) was cloned and expressed as soluble form in Escherichia coli. The recombinant protein was purified with Ni-NTA agarose column and named as vastatin. It inhibited the proliferation of bovine aortic endothelial (BAE) cell stimulated by basic fibroblast growth factor (bFGF) in a dose-dependent manner. The ED50 of vastatin was 0.6 μg/ml, while the ED50 of endostatin was 0.5 μg/ml. Treatment of BAE cell with vastatin caused G0-G1 arrest and cell apoptosis. It is interesting that sequence analysis showed that there was only about 12% amino acid sequence homology between vastatin and endostatin. The structure - function relationship of these angiogenesis molecules remains to be elucidated.
KW - Angiogenesis inhibitor
KW - Apoptosis
KW - NC1 domain
KW - Type VIII collagen
KW - Vastatin
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U2 - 10.1006/bbrc.2001.5970
DO - 10.1006/bbrc.2001.5970
M3 - Article
C2 - 11708810
AN - SCOPUS:0035940970
SN - 0006-291X
VL - 289
SP - 264
EP - 268
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -