Near-infrared fluorescence endoscopy to detect dysplastic lesions in the mouse colon

Elias Gounaris; Yasushige Ishihara; Manisha Shrivastrav; David Bentrem; Terrence A. Barrett

doi:10.1007/978-1-4939-3603-8_13

Near-infrared fluorescence endoscopy to detect dysplastic lesions in the mouse colon

Elias Gounaris, Yasushige Ishihara, Manisha Shrivastrav, David Bentrem, Terrence A. Barrett

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Near-infrared fluorescence (NIRF) endoscopy has a great potential for efficient early detection of dysplastic lesions in the colon. For preclinical studies, we developed a small animal NIRF endoscope and successfully used this device to identify dysplastic lesions in a murine model of chronic colitis. In this chapter, we present a step-by-step protocol for using NIRF endoscopy to examine the location, the size, and the borders of the dysplastic lesions developed in murine colitis. Our studies suggest that NIRF endoscopy is a specific and sensitive technique that provides a unique opportunity to analyze early stages of tumorigenesis in animal models of colon cancer and to perform surveillance colonoscopy in patients with colitisassociated colon cancer.

Original language	English
Pages (from-to)	137-147
Number of pages	11
Journal	Methods in Molecular Biology
Volume	1422
DOIs	https://doi.org/10.1007/978-1-4939-3603-8_13
State	Published - 2016

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2016.

Keywords

Cathepsin activity
Colon cancer
Endoscopy
Mouse colon dysplasia
Mucosal inflammation
Near-infrared probes

ASJC Scopus subject areas

Molecular Biology
Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-4939-3603-8_13

Cite this

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title = "Near-infrared fluorescence endoscopy to detect dysplastic lesions in the mouse colon",

abstract = "Near-infrared fluorescence (NIRF) endoscopy has a great potential for efficient early detection of dysplastic lesions in the colon. For preclinical studies, we developed a small animal NIRF endoscope and successfully used this device to identify dysplastic lesions in a murine model of chronic colitis. In this chapter, we present a step-by-step protocol for using NIRF endoscopy to examine the location, the size, and the borders of the dysplastic lesions developed in murine colitis. Our studies suggest that NIRF endoscopy is a specific and sensitive technique that provides a unique opportunity to analyze early stages of tumorigenesis in animal models of colon cancer and to perform surveillance colonoscopy in patients with colitisassociated colon cancer.",

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AU - Gounaris, Elias

AU - Ishihara, Yasushige

AU - Shrivastrav, Manisha

AU - Bentrem, David

AU - Barrett, Terrence A.

N1 - Publisher Copyright: © Springer Science+Business Media New York 2016.

PY - 2016

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N2 - Near-infrared fluorescence (NIRF) endoscopy has a great potential for efficient early detection of dysplastic lesions in the colon. For preclinical studies, we developed a small animal NIRF endoscope and successfully used this device to identify dysplastic lesions in a murine model of chronic colitis. In this chapter, we present a step-by-step protocol for using NIRF endoscopy to examine the location, the size, and the borders of the dysplastic lesions developed in murine colitis. Our studies suggest that NIRF endoscopy is a specific and sensitive technique that provides a unique opportunity to analyze early stages of tumorigenesis in animal models of colon cancer and to perform surveillance colonoscopy in patients with colitisassociated colon cancer.

AB - Near-infrared fluorescence (NIRF) endoscopy has a great potential for efficient early detection of dysplastic lesions in the colon. For preclinical studies, we developed a small animal NIRF endoscope and successfully used this device to identify dysplastic lesions in a murine model of chronic colitis. In this chapter, we present a step-by-step protocol for using NIRF endoscopy to examine the location, the size, and the borders of the dysplastic lesions developed in murine colitis. Our studies suggest that NIRF endoscopy is a specific and sensitive technique that provides a unique opportunity to analyze early stages of tumorigenesis in animal models of colon cancer and to perform surveillance colonoscopy in patients with colitisassociated colon cancer.

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KW - Colon cancer

KW - Endoscopy

KW - Mouse colon dysplasia

KW - Mucosal inflammation

KW - Near-infrared probes

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JO - Methods in Molecular Biology

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Near-infrared fluorescence endoscopy to detect dysplastic lesions in the mouse colon

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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