Near-infrared spectrometric determination of hydrogen ion, glucose, and human serum albumin in a simulated biological matrix

Near-IR spectroscopy is used for the determination of pH and glucose and human serum albumin concentrations in a simulated biol. matrix. Aqueous samples were equilibrated thermally with a heat exchanger prior to anal. in the spectrometer. The liquid samples were analyzed using math. techniques in which spectra were correlated with known analyte concentrations The prediction equations were evaluated using two criteria: the strength of these correlations and the ability of the prediction equations to calculate concentrations of unknown solutions Good correlations between near-IR spectra and analytes were observed at concentrations an order of magnitude below those ordinarily considered amenable to near-IR anal.