Near-Infrared Spectroscopic Determination of Residual Moisture in Lyophilized Sucrose Through Intact Glass Vials

Madhav S. Kamat, Robert A. Lodder, Patrick P. DeLuca

Research output: Contribution to journalArticlepeer-review

94 Scopus citations

Abstract

A rapid, noninvasive, and nondestructive method for determining moisture in sealed freeze-drying vials is described. The method, based on near-infrared spectrometry, used a novel fiber-optic diffuse-reflectance probe to make remote reflectance measurements from 1100 to 2500 nm through the bottom of glass vials. The correlation of the method to results obtained by Karl Fischer analysis was good (r2 = 0.97). The moisture content of sucrose, a common cryoprotectant, was determined with an error of 0.27% using a single sample scan.

Original languageEnglish
Pages (from-to)961-965
Number of pages5
JournalPharmaceutical Research
Volume6
Issue number11
DOIs
StatePublished - Nov 1989

Keywords

  • fiber-optic
  • freeze-drying
  • sucrose
  • water

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry
  • Pharmacology (medical)

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