Neospora caninum antibodies detected in Midwestern white-tailed deer (Odocoileus virginianus) by Western blot and ELISA

Todd Anderson, Amanda DeJardin, Daniel K. Howe, J. P. Dubey, Michelle L. Michalski

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

White-tailed deer (Odocoileus virginianus) serve to maintain the Neospora caninum life cycle in the wild. Sera from white-tailed deer from south central Wisconsin and southeastern Missouri, USA were tested for antibodies to N. caninum by Western blot analyses and two indirect ELISAs. Seroreactivity against N. caninum surface antigens was observed in 30 of 147 (20%) of WI deer and 11 of 23 (48%) of MO deer using Western blot analysis. Compared to Western blot, the two indirect ELISAs were found to be uninformative due to degradation of the field-collected samples. The results indicate the existence of N. caninum antibodies in MO and WI deer, and that Western blot is superior to ELISA for serologic testing when using degraded blood samples collected from deer carcasses.

Original languageEnglish
Pages (from-to)152-155
Number of pages4
JournalVeterinary Parasitology
Volume145
Issue number1-2
DOIs
StatePublished - Apr 10 2007

Bibliographical note

Funding Information:
The authors thank the WI Department of Natural Resources and the MO Department of Conservation for permission to collect blood samples, and Milt McAllister for sera used in assay development. This work was supported by a grant from the University of Wisconsin Oshkosh Faculty Development Program.

Keywords

  • ELISA
  • Missouri
  • Neospora caninum
  • Seropositivity
  • Western blot
  • White-tailed deer (Odocoileus virginianus)
  • Wisconsin

ASJC Scopus subject areas

  • Parasitology
  • General Veterinary

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