TY - JOUR
T1 - Neuron-Specific mRNA Complexity Responses during Hippocampal Apoptosis after Traumatic Brain Injury
AU - Marciano, Paolo G.
AU - Brettschneider, Julia
AU - Manduchi, Elisabetta
AU - Davis, Jason E.
AU - Eastman, Scott
AU - Raghupathi, Ramesh
AU - Saatman, Kathryn E.
AU - Speed, Terence P.
AU - Stoeckert, Christian J.
AU - Eberwine, James H.
AU - McIntosh, Tracy K.
PY - 2004/3/24
Y1 - 2004/3/24
N2 - In an effort to understand the complexity of genomic responses within selectively vulnerable regions after experimental brain injury, we examined whether single apoptotic neurons from both the CA3 and dentate differed from those in an uninjured brain. The mRNA from individual active caspase 3(+)/terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling [TUNEL(-)] and active caspase 3(+)/TUNEL(+) pyramidal and granule neurons in brain-injured mice were amplified and compared with those from nonlabeled neurons in uninjured brains. Gene analysis revealed that overall expression of mRNAs increased with activation of caspase 3 and decreased to below uninjured levels with TUNEL reactivity. Cell type specificity of the apoptotic response was observed with both regionally distinct expression of mRNAs and differences in those mRNAs that were maximally regulated. Immunohistochemical analysis for two of the most highly differentially expressed genes (prion and Sos2) demonstrated a correlation between the observed differential gene expression after traumatic brain injury and corresponding protein translation.
AB - In an effort to understand the complexity of genomic responses within selectively vulnerable regions after experimental brain injury, we examined whether single apoptotic neurons from both the CA3 and dentate differed from those in an uninjured brain. The mRNA from individual active caspase 3(+)/terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling [TUNEL(-)] and active caspase 3(+)/TUNEL(+) pyramidal and granule neurons in brain-injured mice were amplified and compared with those from nonlabeled neurons in uninjured brains. Gene analysis revealed that overall expression of mRNAs increased with activation of caspase 3 and decreased to below uninjured levels with TUNEL reactivity. Cell type specificity of the apoptotic response was observed with both regionally distinct expression of mRNAs and differences in those mRNAs that were maximally regulated. Immunohistochemical analysis for two of the most highly differentially expressed genes (prion and Sos2) demonstrated a correlation between the observed differential gene expression after traumatic brain injury and corresponding protein translation.
KW - Apoptosis
KW - CA3
KW - Cell death
KW - Dentate
KW - Differential expression
KW - Hippocampus
KW - Microarray
KW - Real-time quantitative PCR
KW - Single-cell amplification
KW - Traumatic brain injury
UR - http://www.scopus.com/inward/record.url?scp=12144288766&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=12144288766&partnerID=8YFLogxK
U2 - 10.1523/JNEUROSCI.5051-03.2004
DO - 10.1523/JNEUROSCI.5051-03.2004
M3 - Article
C2 - 15044525
AN - SCOPUS:12144288766
SN - 0270-6474
VL - 24
SP - 2866
EP - 2876
JO - Journal of Neuroscience
JF - Journal of Neuroscience
IS - 12
ER -