Neuron-Specific mRNA Complexity Responses during Hippocampal Apoptosis after Traumatic Brain Injury

Paolo G. Marciano, Julia Brettschneider, Elisabetta Manduchi, Jason E. Davis, Scott Eastman, Ramesh Raghupathi, Kathryn E. Saatman, Terence P. Speed, Christian J. Stoeckert, James H. Eberwine, Tracy K. McIntosh

Research output: Contribution to journalArticlepeer-review

41 Scopus citations


In an effort to understand the complexity of genomic responses within selectively vulnerable regions after experimental brain injury, we examined whether single apoptotic neurons from both the CA3 and dentate differed from those in an uninjured brain. The mRNA from individual active caspase 3(+)/terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling [TUNEL(-)] and active caspase 3(+)/TUNEL(+) pyramidal and granule neurons in brain-injured mice were amplified and compared with those from nonlabeled neurons in uninjured brains. Gene analysis revealed that overall expression of mRNAs increased with activation of caspase 3 and decreased to below uninjured levels with TUNEL reactivity. Cell type specificity of the apoptotic response was observed with both regionally distinct expression of mRNAs and differences in those mRNAs that were maximally regulated. Immunohistochemical analysis for two of the most highly differentially expressed genes (prion and Sos2) demonstrated a correlation between the observed differential gene expression after traumatic brain injury and corresponding protein translation.

Original languageEnglish
Pages (from-to)2866-2876
Number of pages11
JournalJournal of Neuroscience
Issue number12
StatePublished - Mar 24 2004


  • Apoptosis
  • CA3
  • Cell death
  • Dentate
  • Differential expression
  • Hippocampus
  • Microarray
  • Real-time quantitative PCR
  • Single-cell amplification
  • Traumatic brain injury

ASJC Scopus subject areas

  • Neuroscience (all)


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