Neurturin protects against 6-hydroxydopamine-induced reductions in evoked dopamine overflow in rat striatum

Wayne A. Cass, Laura E. Peters

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Neurturin (NTN), a member of the glial cell line-derived neurotrophic factor (GDNF) family, has substantial effects on normal and lesioned nigrostriatal dopamine systems. However, its ability to protect against toxin-induced loss of striatal dopamine release has not been previously reported. The goal of the present study was to determine if NTN could protect against 6-hydroxydopamine (6-OHDA)-induced reductions in striatal dopamine overflow and tissue levels of dopamine and to compare the effects of NTN with those of GDNF. Male Fischer-344 rats were given a single injection of vehicle, or 5μg NTN or GDNF, into the right striatum. The following day the animals were given a single injection of 12μg 6-OHDA into the striatum at the same site where the trophic factor was injected. Microdialysis experiments conducted three weeks later indicated that the 6-OHDA decreased basal levels of dopamine and metabolites in the lesioned striatum compared to the contralateral striatum, and NTN was able to partially protect against the 6-OHDA-induced reductions. Injection of NTN one day prior to 6-OHDA also led to significant protection against loss of both potassium- and amphetamine-evoked overflow of dopamine. The NTN treatments partially protected against 6-OHDA-induced reductions in striatal tissue levels of dopamine and completely protected against loss of nigral dopamine content. The protective effects of NTN were similar in magnitude to those of GDNF. These results support that within the experimental parameters used in this study, NTN is as effective as GDNF in protecting against the dopamine-depleting effects of intrastriatal 6-OHDA.

Original languageEnglish
Pages (from-to)540-546
Number of pages7
JournalNeurochemistry International
Issue number5
StatePublished - Nov 2010

Bibliographical note

Funding Information:
This study was supported in part by the United States Public Health Service Grant AG17963 . Neither of the authors have a conflict of interest of any type in association with this work.


  • 6-OHDA
  • Dopamine
  • GDNF
  • Microdialysis
  • Neurturin
  • Striatum
  • Substantia Nigra

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology


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