TY - JOUR
T1 - Neutrophil IL-10 suppresses peritoneal inflammatory monocytes during polymicrobial sepsis
AU - Ocuin, Lee M.
AU - Bamboat, Zubin M.
AU - Balachandran, Vinod P.
AU - Cavnar, Michael J.
AU - Obaid, Hebroon
AU - Plitas, George
AU - DeMatteo, Ronald P.
PY - 2011/3
Y1 - 2011/3
N2 - Septic peritonitis remains a major cause of death. Neutrophils and inflammatory monocytes are principal components of the innate immune system and are essential for defense against a range of microbial pathogens. Their role and interaction in polymicrobial sepsis have not been defined clearly. Using a murine model of CLP to induce moderate sepsis, we found that neutrophil depletion did not alter survival, whereas depletion of neutrophils and inflammatory monocytes markedly reduced survival. After neutrophil depletion, inflammatory monocytes had greater phagocytic capacity and oxidative burst, and increased expression of costimulatory molecules, TNF, and iNOS. Notably, peritoneal neutrophils produced IL-10 following CLP. Adoptive i.p. transfer of WT but not IL-10-/- neutrophils into septic mice reduced monocyte expression of TNF. In vitro experiments confirmed that monocyte suppression was mediated by neutrophil-derived IL-10. Thus, during septic peritonitis, neutrophils suppress peritoneal inflammatory monocytes through IL-10 and are dispensable for survival.
AB - Septic peritonitis remains a major cause of death. Neutrophils and inflammatory monocytes are principal components of the innate immune system and are essential for defense against a range of microbial pathogens. Their role and interaction in polymicrobial sepsis have not been defined clearly. Using a murine model of CLP to induce moderate sepsis, we found that neutrophil depletion did not alter survival, whereas depletion of neutrophils and inflammatory monocytes markedly reduced survival. After neutrophil depletion, inflammatory monocytes had greater phagocytic capacity and oxidative burst, and increased expression of costimulatory molecules, TNF, and iNOS. Notably, peritoneal neutrophils produced IL-10 following CLP. Adoptive i.p. transfer of WT but not IL-10-/- neutrophils into septic mice reduced monocyte expression of TNF. In vitro experiments confirmed that monocyte suppression was mediated by neutrophil-derived IL-10. Thus, during septic peritonitis, neutrophils suppress peritoneal inflammatory monocytes through IL-10 and are dispensable for survival.
KW - Bacteria
KW - Cecal ligation and puncture
KW - Cytokines
KW - Innate immunity
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U2 - 10.1189/jlb.0810479
DO - 10.1189/jlb.0810479
M3 - Article
C2 - 21106642
AN - SCOPUS:79952205667
SN - 0741-5400
VL - 89
SP - 423
EP - 432
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
IS - 3
ER -