New cosmid vectors for library construction, chromosome walking and restriction mapping in filamentous fungi

Zhiqiang An; Mark L. Farman; Allen Budde; Satoru Taura; Sally A. Leong

doi:10.1016/0378-1119(96)00225-9

New cosmid vectors for library construction, chromosome walking and restriction mapping in filamentous fungi

Zhiqiang An, Mark L. Farman, Allen Budde, Satoru Taura, Sally A. Leong

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

New cosmid vectors were constructed for the ascomycete fungus, Magnaporthe grisea and the basidiomycete fungus, Ustilago maydis. These vectors are capable of transforming M. grisea at frequencies of up to 5 transformants/μg linear DNA and U. maydis at up to 25 transformants/μg circular DNA for integrative transformation. In addition, 2800 transformants/μg DNA are possible when using an autonomously replicating vector. Since the promoters used in these vectors function in other ascomycete and basidiomycete fungi, we anticipate that these vectors will be widely applicable.

Original language	English
Pages (from-to)	93-96
Number of pages	4
Journal	Gene
Volume	176
Issue number	1-2
DOIs	https://doi.org/10.1016/0378-1119(96)00225-9
State	Published - Oct 17 1996

Keywords

Ascomycetes
Basidiomycetes
Promoter
Transformant

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(96)00225-9

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abstract = "New cosmid vectors were constructed for the ascomycete fungus, Magnaporthe grisea and the basidiomycete fungus, Ustilago maydis. These vectors are capable of transforming M. grisea at frequencies of up to 5 transformants/μg linear DNA and U. maydis at up to 25 transformants/μg circular DNA for integrative transformation. In addition, 2800 transformants/μg DNA are possible when using an autonomously replicating vector. Since the promoters used in these vectors function in other ascomycete and basidiomycete fungi, we anticipate that these vectors will be widely applicable.",

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T1 - New cosmid vectors for library construction, chromosome walking and restriction mapping in filamentous fungi

AU - An, Zhiqiang

AU - Farman, Mark L.

AU - Budde, Allen

AU - Taura, Satoru

AU - Leong, Sally A.

PY - 1996/10/17

Y1 - 1996/10/17

N2 - New cosmid vectors were constructed for the ascomycete fungus, Magnaporthe grisea and the basidiomycete fungus, Ustilago maydis. These vectors are capable of transforming M. grisea at frequencies of up to 5 transformants/μg linear DNA and U. maydis at up to 25 transformants/μg circular DNA for integrative transformation. In addition, 2800 transformants/μg DNA are possible when using an autonomously replicating vector. Since the promoters used in these vectors function in other ascomycete and basidiomycete fungi, we anticipate that these vectors will be widely applicable.

AB - New cosmid vectors were constructed for the ascomycete fungus, Magnaporthe grisea and the basidiomycete fungus, Ustilago maydis. These vectors are capable of transforming M. grisea at frequencies of up to 5 transformants/μg linear DNA and U. maydis at up to 25 transformants/μg circular DNA for integrative transformation. In addition, 2800 transformants/μg DNA are possible when using an autonomously replicating vector. Since the promoters used in these vectors function in other ascomycete and basidiomycete fungi, we anticipate that these vectors will be widely applicable.

KW - Ascomycetes

KW - Basidiomycetes

KW - Promoter

KW - Transformant

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New cosmid vectors for library construction, chromosome walking and restriction mapping in filamentous fungi

Abstract

Keywords

ASJC Scopus subject areas

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