Next-Generation sequencing reveals a controlled immune response to Zaire Ebola virus challenge in cynomolgus macaques immunized with vesicular stomatitis virus expressing Zaire Ebola virus glycoprotein (VSVΔG/EBOVgp)

Fredrik Barrenas; Richard R. Green; Matthew J. Thomas; G. Lynn Law; Sean C. Proll; Flora Engelmann; Ilhem Messaoudi; Andrea Marzi; Heinz Feldmann; Michael G. Katzea

doi:10.1128/CVI.00733-14

Next-Generation sequencing reveals a controlled immune response to Zaire Ebola virus challenge in cynomolgus macaques immunized with vesicular stomatitis virus expressing Zaire Ebola virus glycoprotein (VSVΔG/EBOVgp)

Fredrik Barrenas, Richard R. Green, Matthew J. Thomas, G. Lynn Law, Sean C. Proll, Flora Engelmann, Ilhem Messaoudi, Andrea Marzi, Heinz Feldmann, Michael G. Katzea

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Vesicularstomatitis virus expressing Zaire Ebola virus (EBOV) glycoprotein (VSVΔG/EBOVgp) could be used as a vaccine to meet the 2014 Ebola virus outbreak. To characterize the host response to this vaccine, we used mRNA sequencing to analyze peripheral blood mononuclear cells (PBMCs) from cynomolgus macaques after VSVΔG/EBOVgp immunization and subsequent EBOV challenge. We found a controlled transcriptional response that transitioned to immune regulation as the EBOV was cleared. This observation supports the safety of the vaccine.

Original language	English
Pages (from-to)	354-356
Number of pages	3
Journal	Clinical and Vaccine Immunology
Volume	22
Issue number	3
DOIs	https://doi.org/10.1128/CVI.00733-14
State	Published - Mar 1 2015

Bibliographical note

Publisher Copyright:
Copyright © 2015, American Society for Microbiology. All Rights Reserved.

Funding

Funders	Funder number
National Institute of Allergy and Infectious Diseases	ZIAAI001163

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Clinical Biochemistry
Microbiology (medical)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/CVI.00733-14

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Barrenas, F., Green, R. R., Thomas, M. J., Law, G. L., Proll, S. C., Engelmann, F., Messaoudi, I., Marzi, A., Feldmann, H., & Katzea, M. G. (2015). Next-Generation sequencing reveals a controlled immune response to Zaire Ebola virus challenge in cynomolgus macaques immunized with vesicular stomatitis virus expressing Zaire Ebola virus glycoprotein (VSVΔG/EBOVgp). Clinical and Vaccine Immunology, 22(3), 354-356. https://doi.org/10.1128/CVI.00733-14

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abstract = "Vesicularstomatitis virus expressing Zaire Ebola virus (EBOV) glycoprotein (VSVΔG/EBOVgp) could be used as a vaccine to meet the 2014 Ebola virus outbreak. To characterize the host response to this vaccine, we used mRNA sequencing to analyze peripheral blood mononuclear cells (PBMCs) from cynomolgus macaques after VSVΔG/EBOVgp immunization and subsequent EBOV challenge. We found a controlled transcriptional response that transitioned to immune regulation as the EBOV was cleared. This observation supports the safety of the vaccine.",

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Barrenas, F, Green, RR, Thomas, MJ, Law, GL, Proll, SC, Engelmann, F, Messaoudi, I, Marzi, A, Feldmann, H & Katzea, MG 2015, 'Next-Generation sequencing reveals a controlled immune response to Zaire Ebola virus challenge in cynomolgus macaques immunized with vesicular stomatitis virus expressing Zaire Ebola virus glycoprotein (VSVΔG/EBOVgp)', Clinical and Vaccine Immunology, vol. 22, no. 3, pp. 354-356. https://doi.org/10.1128/CVI.00733-14

Next-Generation sequencing reveals a controlled immune response to Zaire Ebola virus challenge in cynomolgus macaques immunized with vesicular stomatitis virus expressing Zaire Ebola virus glycoprotein (VSVΔG/EBOVgp). / Barrenas, Fredrik; Green, Richard R.; Thomas, Matthew J. et al.
In: Clinical and Vaccine Immunology, Vol. 22, No. 3, 01.03.2015, p. 354-356.

Research output: Contribution to journal › Article › peer-review

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Barrenas F, Green RR, Thomas MJ, Law GL, Proll SC, Engelmann F et al. Next-Generation sequencing reveals a controlled immune response to Zaire Ebola virus challenge in cynomolgus macaques immunized with vesicular stomatitis virus expressing Zaire Ebola virus glycoprotein (VSVΔG/EBOVgp). Clinical and Vaccine Immunology. 2015 Mar 1;22(3):354-356. doi: 10.1128/CVI.00733-14

Next-Generation sequencing reveals a controlled immune response to Zaire Ebola virus challenge in cynomolgus macaques immunized with vesicular stomatitis virus expressing Zaire Ebola virus glycoprotein (VSVΔG/EBOVgp)

Abstract

Bibliographical note

Funding

ASJC Scopus subject areas

UN SDGs

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