Nicotine attenuates arachidonic acid-induced neurotoxicity in cultured spinal cord neurons

Rosario Garrido, Andrzej Malecki, Bernhard Hennig, Michal Toborek

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


Arachidonic acid release from cellular membranes due to spinal cord trauma may be one of the principal destructive events that can lead to progressive injury to spinal cord tissue. Exposure to arachidonic acid can compromise neuronal survival and viability. Because nicotine is known to be a neuroprotective agent, we propose that it can prevent arachidonic acid- induced neurotoxicity. To study this hypothesis, effects of nicotine on mitochondrial function, cellular energy content and apoptotic cell death were measured in cultured spinal cord neurons treated with arachidonic acid. Nicotine attenuated arachidonic acid-induced compromised cell viability and cellular ATP levels in spinal cord neurons. Nicotine exerted these protective effects when used at the concentration of 10 μM and only after a 2-h pre- treatment before a co-exposure to arachidonic acid. Antagonists of nicotinic receptors, such as α-bungarotoxin or mecamylamine, only partially reversed these neuroprotective effects of nicotine. In addition, nicotine prevented arachidonic acid-induced activation of caspase-3 activity and apoptotic cell death. These results indicate that nicotine pre-treatment can exert a protective effect against arachidonic acid-induced injury to spinal cord neurons. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)59-68
Number of pages10
JournalBrain Research
Issue number1
StatePublished - Apr 7 2000

Bibliographical note

Funding Information:
This work was supported in part by THRI and KSCHIRT. Fluorescent measurements of caspase-3 activity were performed at the University of Kentucky Confocal Scanning Microscopy Facilities (Dr. Mark P. Mattson, Director).


  • Apoptosis
  • Free fatty acid
  • Nicotinic receptor
  • Spinal cord trauma

ASJC Scopus subject areas

  • General Neuroscience
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


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