Nicotine-evoked nitric oxide release in the rat hippocampal slice

Dennison A. Smith; Alexander F. Hoffman; Daniel J. David; Catherine E. Adams; Greg A. Gerhardt

doi:10.1016/S0304-3940(98)00725-3

Nicotine-evoked nitric oxide release in the rat hippocampal slice

Dennison A. Smith, Alexander F. Hoffman, Daniel J. David, Catherine E. Adams, Greg A. Gerhardt

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

The effects of cholinergic agonists on nitric oxide (NO) release in hippocampal slices from male Sprague-Dawley rats were investigated using electrochemical recording procedures using Nafion and o-phenylenediamine- treated carbon fiber microelectrodes. These microelectrodes are highly selective for NO versus other interferents. Acetylcholine (Ach) with neostigmine, or nicotine was delivered by pressure ejection from pipettes placed within 300 μm of the NO sensors. Both Ach arid nicotine produced NO signals ranging from 0.04 to 2.14 μM in the CA1, CA3, and dentate gyrus of the rat hippocampus that lasted for 2-5 min. The Ach responses were not antagonized by the muscarinic antagonist atropine. However, nicotine-evoked responses were partially antagonized by α-bungarotoxin, a finding consistent with α7-nicotinic cholinergic receptors being involved with the effects of nicotine. These data support the hypothesis that nicotine is capable of evoking long lasting NO release in the hippocampus.

Original language	English
Pages (from-to)	127-130
Number of pages	4
Journal	Neuroscience Letters
Volume	255
Issue number	3
DOIs	https://doi.org/10.1016/S0304-3940(98)00725-3
State	Published - Oct 23 1998

Bibliographical note

Funding Information:
This work was support by USPHS grants AG06434, NS09199 and a Level II Research Scientist Development Award MH01245 to G.A.G. In addition, A.F.H. is the recipient of an Advanced Predoctoral Fellowship from the Pharmaceutical Research and Manufacturers of America Foundation.

Keywords

Acetylcholine
Brain slices
Hippocampus
Nicotine
Nitric oxide
Rat
Schizophrenia

ASJC Scopus subject areas

General Neuroscience

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10.1016/S0304-3940(98)00725-3

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title = "Nicotine-evoked nitric oxide release in the rat hippocampal slice",

abstract = "The effects of cholinergic agonists on nitric oxide (NO) release in hippocampal slices from male Sprague-Dawley rats were investigated using electrochemical recording procedures using Nafion and o-phenylenediamine- treated carbon fiber microelectrodes. These microelectrodes are highly selective for NO versus other interferents. Acetylcholine (Ach) with neostigmine, or nicotine was delivered by pressure ejection from pipettes placed within 300 μm of the NO sensors. Both Ach arid nicotine produced NO signals ranging from 0.04 to 2.14 μM in the CA1, CA3, and dentate gyrus of the rat hippocampus that lasted for 2-5 min. The Ach responses were not antagonized by the muscarinic antagonist atropine. However, nicotine-evoked responses were partially antagonized by α-bungarotoxin, a finding consistent with α7-nicotinic cholinergic receptors being involved with the effects of nicotine. These data support the hypothesis that nicotine is capable of evoking long lasting NO release in the hippocampus.",

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note = "Funding Information: This work was support by USPHS grants AG06434, NS09199 and a Level II Research Scientist Development Award MH01245 to G.A.G. In addition, A.F.H. is the recipient of an Advanced Predoctoral Fellowship from the Pharmaceutical Research and Manufacturers of America Foundation.",

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AU - David, Daniel J.

AU - Adams, Catherine E.

AU - Gerhardt, Greg A.

N1 - Funding Information: This work was support by USPHS grants AG06434, NS09199 and a Level II Research Scientist Development Award MH01245 to G.A.G. In addition, A.F.H. is the recipient of an Advanced Predoctoral Fellowship from the Pharmaceutical Research and Manufacturers of America Foundation.

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N2 - The effects of cholinergic agonists on nitric oxide (NO) release in hippocampal slices from male Sprague-Dawley rats were investigated using electrochemical recording procedures using Nafion and o-phenylenediamine- treated carbon fiber microelectrodes. These microelectrodes are highly selective for NO versus other interferents. Acetylcholine (Ach) with neostigmine, or nicotine was delivered by pressure ejection from pipettes placed within 300 μm of the NO sensors. Both Ach arid nicotine produced NO signals ranging from 0.04 to 2.14 μM in the CA1, CA3, and dentate gyrus of the rat hippocampus that lasted for 2-5 min. The Ach responses were not antagonized by the muscarinic antagonist atropine. However, nicotine-evoked responses were partially antagonized by α-bungarotoxin, a finding consistent with α7-nicotinic cholinergic receptors being involved with the effects of nicotine. These data support the hypothesis that nicotine is capable of evoking long lasting NO release in the hippocampus.

AB - The effects of cholinergic agonists on nitric oxide (NO) release in hippocampal slices from male Sprague-Dawley rats were investigated using electrochemical recording procedures using Nafion and o-phenylenediamine- treated carbon fiber microelectrodes. These microelectrodes are highly selective for NO versus other interferents. Acetylcholine (Ach) with neostigmine, or nicotine was delivered by pressure ejection from pipettes placed within 300 μm of the NO sensors. Both Ach arid nicotine produced NO signals ranging from 0.04 to 2.14 μM in the CA1, CA3, and dentate gyrus of the rat hippocampus that lasted for 2-5 min. The Ach responses were not antagonized by the muscarinic antagonist atropine. However, nicotine-evoked responses were partially antagonized by α-bungarotoxin, a finding consistent with α7-nicotinic cholinergic receptors being involved with the effects of nicotine. These data support the hypothesis that nicotine is capable of evoking long lasting NO release in the hippocampus.

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KW - Nitric oxide

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KW - Schizophrenia

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Nicotine-evoked nitric oxide release in the rat hippocampal slice

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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