(-)-Nicotine increases mRNA encoding G3PDH and the vesicular acetylcholine transporter in vivo

Mark A. Prendergast, Jerry J. Buccafusco

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The effects of acute in vivo (-)-nicotine exposure on neuronal expression of mRNA encoding the vesicular acetylcholine transport (VAChT) and the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (G3PDH) were examined. The reverse transcription polymerase chain reaction was used to amplify cDNA derived from brain tissue of adult male rats injected acutely with (-)-nicotine and semi-quantification of cDNA products conducted using anion exchange chromatography. In cerebral cortex, levels of cDNA derived from the VAChT mRNA were increased by > 65% above control levels 1 h after (- )-nicotine (0.8 mg/kg, s.c.) exposure. In hippocampal samples, levels of this cDNA were increased by 15% above control levels. cDNA derived from G3PDH mRNA was increased by 86% and 280% above control levels in the cerebral cortex and hippocampus, respectively. Thus, increased expression and function of proteins associated with these mRNA species are likely consequences of acute (-)-nicotine exposure.

Original languageEnglish
Pages (from-to)1385-1389
Number of pages5
JournalNeuroReport
Volume9
Issue number7
DOIs
StatePublished - May 11 1998

Keywords

  • Acetylcholine
  • G3PDH
  • Gene expression
  • mRNA
  • Nicotine
  • VAChT

ASJC Scopus subject areas

  • Neuroscience (all)

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