Nitrate-nonutilizing mutants used to study heterokaryosis and vegetative compatibility in Glomerella graminicola (Colletotrichum graminicola)

Lisa J. Vaillancourt, Robert M. Hanau

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Nitrate-nonutilizing (nit) mutants of Glomerella graminicola were recovered by selecting chlorate-resistant sectors. Heterokaryons were formed by complementation between two different classes of nit mutants. Complementation groups were distinguished in nitrogen feeding tests and segregated as two, unlinked genes among random progeny of sexual crosses. The two genes are comparable to those encoding the nitrate reductase enzyme and one of a series of molybdenum cofactors in Aspergillus nidulans and Neurospora crassa. Heterokaryon tests were reliable indicators of allelic and dominance relationships between mutations with similar phenotypes. Vegetative compatibility (VC) between two strains of G. graminicola appeared to be regulated by approximately five unlinked VC loci, analogous to those described for other fungi.

Original languageEnglish
Pages (from-to)311-319
Number of pages9
JournalExperimental Mycology
Volume18
Issue number4
DOIs
StatePublished - 1994

Bibliographical note

Funding Information:
We are grateful to Dr. Ralph Nicholson and Dr. H. P. da Silva for generously providing some of the fungal isolates used in this study. We especially thank Mr. Howard Laffoon for excellent technical assistance. These studies were supported by Grant 90-37262-5445 from the CRGO of the USDA and by tri-agency Grant 92-37310-7821 from the DOE/NSF/ USDA for joint programs on collaborative research in plant biology. This is Journal Paper 13,974 from the Purdue University Agricultural Experiment Station.

Keywords

  • Glomerella graminicola
  • Mel
  • VC
  • anthracnose
  • chlorate resistance
  • heterokaryosis
  • melanin-deficient
  • nit
  • nitrate reductase
  • nitrate-nonutilizing
  • vegetative compatibility
  • vegetative compatibility

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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