Nitrite oxidizing bacteria, Nitrobacter and Nitrospira, are differently influenced by season, fertilizer, and tillage in long-term maize culture

S. Liu; M. S. Coyne; J. H. Grove; M. D. Flythe

doi:10.1016/j.apsoil.2022.104530

Nitrite oxidizing bacteria, Nitrobacter and Nitrospira, are differently influenced by season, fertilizer, and tillage in long-term maize culture

S. Liu, M. S. Coyne, J. H. Grove, M. D. Flythe

Plant and Soil Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Nitrite (NO₂⁻) oxidation is the second step in nitrification, following ammonia (NH₃) oxidation, and catalyzed by nitrite oxidizing bacteria (NOB). Their activity is critical in preventing toxic accumulation of NO₂⁻. The two major NOB genera in soil are Nitrobacter and Nitrospira. This study investigated how N fertilization and tillage management influenced these two NOB communities in long-term (>40 years) maize (Zea mays L.) cropping. To evaluate NOB community changes we used PCR and denaturing gradient gel electrophoresis (DGGE) to analyze Nitrobacter and Nitrospira NO₂⁻ oxidoreductase genes (nxr). Season, fertilizer rate, and tillage all influenced Nitrobacter and Nitrospira communities, but differentially for the two genera. Nitrobacter was more diverse in summer, whereas Nitrospira was more diverse in winter. Nitrobacter was more diverse in N-fertilized samples, whereas Nitrospira diversity decreased with increasing fertilizer rate in winter but not summer. Nitrobacter diversity was not significantly influenced by tillage, whereas no-tillage samples had more diverse Nitrospira, compared to plow tillage samples. In addition to providing evidence for better understanding the relationship between soil management and NOB communities, this study also helped to suggest linkages between ammonia oxidizing bacteria and Nitrobacter and between ammonia oxidizing archaea and Nitrospira that may facilitate future studies concerning ammonia-oxidizing nitrifiers and NOB.

Original language	English
Article number	104530
Journal	Applied Soil Ecology
Volume	177
DOIs	https://doi.org/10.1016/j.apsoil.2022.104530
State	Published - Sep 2022

Bibliographical note

Funding Information:
The investigation reported in this paper is in connection with a project (1024123) of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. Mention of trade names is for information only and does not imply endorsement by the Kentucky Agricultural Experiment Station. S. Liu was assisted by research and fellowship support from the Department of Plant and Soil Sciences and a College of Agriculture, Food, and Environment Research Activities Award (University of Kentucky). The technical supports of Ann Freytag and Gloria Gellin are greatly appreciated.

Funding Information:
The investigation reported in this paper is in connection with a project (1024123) of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. Mention of trade names is for information only and does not imply endorsement by the Kentucky Agricultural Experiment Station. S. Liu was assisted by research and fellowship support from the Department of Plant and Soil Sciences and a College of Agriculture, Food, and Environment Research Activities Award ( University of Kentucky ). The technical supports of Ann Freytag and Gloria Gellin are greatly appreciated.

Publisher Copyright:
© 2022 Elsevier B.V.

Keywords

Community analysis
DGGE
Nitrification
Nitrite oxidation

ASJC Scopus subject areas

Ecology
Agricultural and Biological Sciences (miscellaneous)
Soil Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.apsoil.2022.104530

Cite this

@article{83fbf13ec73b424391263bf0d930235f,

title = "Nitrite oxidizing bacteria, Nitrobacter and Nitrospira, are differently influenced by season, fertilizer, and tillage in long-term maize culture",

abstract = "Nitrite (NO2−) oxidation is the second step in nitrification, following ammonia (NH3) oxidation, and catalyzed by nitrite oxidizing bacteria (NOB). Their activity is critical in preventing toxic accumulation of NO2−. The two major NOB genera in soil are Nitrobacter and Nitrospira. This study investigated how N fertilization and tillage management influenced these two NOB communities in long-term (>40 years) maize (Zea mays L.) cropping. To evaluate NOB community changes we used PCR and denaturing gradient gel electrophoresis (DGGE) to analyze Nitrobacter and Nitrospira NO2− oxidoreductase genes (nxr). Season, fertilizer rate, and tillage all influenced Nitrobacter and Nitrospira communities, but differentially for the two genera. Nitrobacter was more diverse in summer, whereas Nitrospira was more diverse in winter. Nitrobacter was more diverse in N-fertilized samples, whereas Nitrospira diversity decreased with increasing fertilizer rate in winter but not summer. Nitrobacter diversity was not significantly influenced by tillage, whereas no-tillage samples had more diverse Nitrospira, compared to plow tillage samples. In addition to providing evidence for better understanding the relationship between soil management and NOB communities, this study also helped to suggest linkages between ammonia oxidizing bacteria and Nitrobacter and between ammonia oxidizing archaea and Nitrospira that may facilitate future studies concerning ammonia-oxidizing nitrifiers and NOB.",

keywords = "Community analysis, DGGE, Nitrification, Nitrite oxidation",

author = "S. Liu and Coyne, {M. S.} and Grove, {J. H.} and Flythe, {M. D.}",

note = "Funding Information: The investigation reported in this paper is in connection with a project (1024123) of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. Mention of trade names is for information only and does not imply endorsement by the Kentucky Agricultural Experiment Station. S. Liu was assisted by research and fellowship support from the Department of Plant and Soil Sciences and a College of Agriculture, Food, and Environment Research Activities Award (University of Kentucky). The technical supports of Ann Freytag and Gloria Gellin are greatly appreciated. Funding Information: The investigation reported in this paper is in connection with a project (1024123) of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. Mention of trade names is for information only and does not imply endorsement by the Kentucky Agricultural Experiment Station. S. Liu was assisted by research and fellowship support from the Department of Plant and Soil Sciences and a College of Agriculture, Food, and Environment Research Activities Award ( University of Kentucky ). The technical supports of Ann Freytag and Gloria Gellin are greatly appreciated. Publisher Copyright: {\textcopyright} 2022 Elsevier B.V.",

year = "2022",

month = sep,

doi = "10.1016/j.apsoil.2022.104530",

language = "English",

volume = "177",

}

TY - JOUR

T1 - Nitrite oxidizing bacteria, Nitrobacter and Nitrospira, are differently influenced by season, fertilizer, and tillage in long-term maize culture

AU - Liu, S.

AU - Coyne, M. S.

AU - Grove, J. H.

AU - Flythe, M. D.

N1 - Funding Information: The investigation reported in this paper is in connection with a project (1024123) of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. Mention of trade names is for information only and does not imply endorsement by the Kentucky Agricultural Experiment Station. S. Liu was assisted by research and fellowship support from the Department of Plant and Soil Sciences and a College of Agriculture, Food, and Environment Research Activities Award (University of Kentucky). The technical supports of Ann Freytag and Gloria Gellin are greatly appreciated. Funding Information: The investigation reported in this paper is in connection with a project (1024123) of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. Mention of trade names is for information only and does not imply endorsement by the Kentucky Agricultural Experiment Station. S. Liu was assisted by research and fellowship support from the Department of Plant and Soil Sciences and a College of Agriculture, Food, and Environment Research Activities Award ( University of Kentucky ). The technical supports of Ann Freytag and Gloria Gellin are greatly appreciated. Publisher Copyright: © 2022 Elsevier B.V.

PY - 2022/9

Y1 - 2022/9

N2 - Nitrite (NO2−) oxidation is the second step in nitrification, following ammonia (NH3) oxidation, and catalyzed by nitrite oxidizing bacteria (NOB). Their activity is critical in preventing toxic accumulation of NO2−. The two major NOB genera in soil are Nitrobacter and Nitrospira. This study investigated how N fertilization and tillage management influenced these two NOB communities in long-term (>40 years) maize (Zea mays L.) cropping. To evaluate NOB community changes we used PCR and denaturing gradient gel electrophoresis (DGGE) to analyze Nitrobacter and Nitrospira NO2− oxidoreductase genes (nxr). Season, fertilizer rate, and tillage all influenced Nitrobacter and Nitrospira communities, but differentially for the two genera. Nitrobacter was more diverse in summer, whereas Nitrospira was more diverse in winter. Nitrobacter was more diverse in N-fertilized samples, whereas Nitrospira diversity decreased with increasing fertilizer rate in winter but not summer. Nitrobacter diversity was not significantly influenced by tillage, whereas no-tillage samples had more diverse Nitrospira, compared to plow tillage samples. In addition to providing evidence for better understanding the relationship between soil management and NOB communities, this study also helped to suggest linkages between ammonia oxidizing bacteria and Nitrobacter and between ammonia oxidizing archaea and Nitrospira that may facilitate future studies concerning ammonia-oxidizing nitrifiers and NOB.

AB - Nitrite (NO2−) oxidation is the second step in nitrification, following ammonia (NH3) oxidation, and catalyzed by nitrite oxidizing bacteria (NOB). Their activity is critical in preventing toxic accumulation of NO2−. The two major NOB genera in soil are Nitrobacter and Nitrospira. This study investigated how N fertilization and tillage management influenced these two NOB communities in long-term (>40 years) maize (Zea mays L.) cropping. To evaluate NOB community changes we used PCR and denaturing gradient gel electrophoresis (DGGE) to analyze Nitrobacter and Nitrospira NO2− oxidoreductase genes (nxr). Season, fertilizer rate, and tillage all influenced Nitrobacter and Nitrospira communities, but differentially for the two genera. Nitrobacter was more diverse in summer, whereas Nitrospira was more diverse in winter. Nitrobacter was more diverse in N-fertilized samples, whereas Nitrospira diversity decreased with increasing fertilizer rate in winter but not summer. Nitrobacter diversity was not significantly influenced by tillage, whereas no-tillage samples had more diverse Nitrospira, compared to plow tillage samples. In addition to providing evidence for better understanding the relationship between soil management and NOB communities, this study also helped to suggest linkages between ammonia oxidizing bacteria and Nitrobacter and between ammonia oxidizing archaea and Nitrospira that may facilitate future studies concerning ammonia-oxidizing nitrifiers and NOB.

KW - Community analysis

KW - DGGE

KW - Nitrification

KW - Nitrite oxidation

UR - http://www.scopus.com/inward/record.url?scp=85130342590&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85130342590&partnerID=8YFLogxK

U2 - 10.1016/j.apsoil.2022.104530

DO - 10.1016/j.apsoil.2022.104530

M3 - Article

AN - SCOPUS:85130342590

SN - 0929-1393

VL - 177

JO - Applied Soil Ecology

JF - Applied Soil Ecology

M1 - 104530

ER -

Nitrite oxidizing bacteria, Nitrobacter and Nitrospira, are differently influenced by season, fertilizer, and tillage in long-term maize culture

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this