Nitrogen, season, and tillage management influence ammonia oxidizing bacterial communities in long-term maize

S. Liu; M. S. Coyne; J. H. Grove; M. D. Flythe

doi:10.1016/j.apsoil.2018.05.002

Nitrogen, season, and tillage management influence ammonia oxidizing bacterial communities in long-term maize

S. Liu, M. S. Coyne, J. H. Grove, M. D. Flythe

Plant and Soil Sciences

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Nitrification is the biological oxidation of NH₃ to NO₂ ⁻ and NO₃ ⁻ for which ammonia-oxidizing bacteria (AOB) are the functionally dominant group in cropland responsible for the rate-limiting step of NH₃ oxidation. Different AOB species have functional differentiation and do not equally contribute to nitrification. This investigation explored if long-term N fertilization and tillage influenced AOB community structure. The study site was a long-term (>40 years) continuous maize (Zea mays L.) experiment with three N fertilization rates (0, 168, and 336 kg ha⁻¹) and either no-tillage (NT) or moldboard plow tillage (PT). We used denaturing gradient gel electrophoresis (DGGE) to analyze PCR-amplified bacterial ammonia monooxygenase genes and detect changes among NH₃-oxidizing bacteria. Tillage, fertilization, and sample season all significantly influenced the AOB community. The AOB were more diverse in NT than PT. The AOB became more diverse with increasing N input and were more diverse in summer than winter samples. Nitrosomonas-like and Nitrosospira-like groups were identified based on gel migration patterns. Unique bands occurred in different treatments, manifesting environmental selection. The long-term field trial showed soil management consistently influenced AOB communities. Significantly, AOB diversity differed at a small scale within a relatively uniform landscape, even in the presence of long-term management practices.

Original language	English
Pages (from-to)	98-106
Number of pages	9
Journal	Applied Soil Ecology
Volume	129
DOIs	https://doi.org/10.1016/j.apsoil.2018.05.002
State	Published - Aug 2018

Bibliographical note

Funding Information:
The investigation reported in this paper (16-06-051) is in connection with a project of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. Mention of trade names is for information only and does not imply endorsement by the Kentucky Agricultural Experiment Station. S. Liu was assisted by research and fellowship support from the Department of Plant and Soil Sciences and a College of Agriculture, Food, and Environment Research Activities Award (University of Kentucky). The technical support of Ann Freytag and Gloria Gellin are greatly appreciated as are the analytical facilities of the USDA-FAPRU unit. Appendix A

Publisher Copyright:
© 2018 Elsevier B.V.

Keywords

Ammonia oxidizing bacteria
Autotrophic nitrification
Community structure
PCR-DGGE

ASJC Scopus subject areas

Ecology
Agricultural and Biological Sciences (miscellaneous)
Soil Science

Access to Document

10.1016/j.apsoil.2018.05.002

Cite this

@article{c0aeab9538844a77be239296437f3245,

title = "Nitrogen, season, and tillage management influence ammonia oxidizing bacterial communities in long-term maize",

abstract = "Nitrification is the biological oxidation of NH3 to NO2 − and NO3 − for which ammonia-oxidizing bacteria (AOB) are the functionally dominant group in cropland responsible for the rate-limiting step of NH3 oxidation. Different AOB species have functional differentiation and do not equally contribute to nitrification. This investigation explored if long-term N fertilization and tillage influenced AOB community structure. The study site was a long-term (>40 years) continuous maize (Zea mays L.) experiment with three N fertilization rates (0, 168, and 336 kg ha−1) and either no-tillage (NT) or moldboard plow tillage (PT). We used denaturing gradient gel electrophoresis (DGGE) to analyze PCR-amplified bacterial ammonia monooxygenase genes and detect changes among NH3-oxidizing bacteria. Tillage, fertilization, and sample season all significantly influenced the AOB community. The AOB were more diverse in NT than PT. The AOB became more diverse with increasing N input and were more diverse in summer than winter samples. Nitrosomonas-like and Nitrosospira-like groups were identified based on gel migration patterns. Unique bands occurred in different treatments, manifesting environmental selection. The long-term field trial showed soil management consistently influenced AOB communities. Significantly, AOB diversity differed at a small scale within a relatively uniform landscape, even in the presence of long-term management practices.",

keywords = "Ammonia oxidizing bacteria, Autotrophic nitrification, Community structure, PCR-DGGE",

author = "S. Liu and Coyne, {M. S.} and Grove, {J. H.} and Flythe, {M. D.}",

note = "Funding Information: The investigation reported in this paper (16-06-051) is in connection with a project of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. Mention of trade names is for information only and does not imply endorsement by the Kentucky Agricultural Experiment Station. S. Liu was assisted by research and fellowship support from the Department of Plant and Soil Sciences and a College of Agriculture, Food, and Environment Research Activities Award (University of Kentucky). The technical support of Ann Freytag and Gloria Gellin are greatly appreciated as are the analytical facilities of the USDA-FAPRU unit. Appendix A Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

year = "2018",

month = aug,

doi = "10.1016/j.apsoil.2018.05.002",

language = "English",

volume = "129",

pages = "98--106",

}

TY - JOUR

T1 - Nitrogen, season, and tillage management influence ammonia oxidizing bacterial communities in long-term maize

AU - Liu, S.

AU - Coyne, M. S.

AU - Grove, J. H.

AU - Flythe, M. D.

N1 - Funding Information: The investigation reported in this paper (16-06-051) is in connection with a project of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. Mention of trade names is for information only and does not imply endorsement by the Kentucky Agricultural Experiment Station. S. Liu was assisted by research and fellowship support from the Department of Plant and Soil Sciences and a College of Agriculture, Food, and Environment Research Activities Award (University of Kentucky). The technical support of Ann Freytag and Gloria Gellin are greatly appreciated as are the analytical facilities of the USDA-FAPRU unit. Appendix A Publisher Copyright: © 2018 Elsevier B.V.

PY - 2018/8

Y1 - 2018/8

N2 - Nitrification is the biological oxidation of NH3 to NO2 − and NO3 − for which ammonia-oxidizing bacteria (AOB) are the functionally dominant group in cropland responsible for the rate-limiting step of NH3 oxidation. Different AOB species have functional differentiation and do not equally contribute to nitrification. This investigation explored if long-term N fertilization and tillage influenced AOB community structure. The study site was a long-term (>40 years) continuous maize (Zea mays L.) experiment with three N fertilization rates (0, 168, and 336 kg ha−1) and either no-tillage (NT) or moldboard plow tillage (PT). We used denaturing gradient gel electrophoresis (DGGE) to analyze PCR-amplified bacterial ammonia monooxygenase genes and detect changes among NH3-oxidizing bacteria. Tillage, fertilization, and sample season all significantly influenced the AOB community. The AOB were more diverse in NT than PT. The AOB became more diverse with increasing N input and were more diverse in summer than winter samples. Nitrosomonas-like and Nitrosospira-like groups were identified based on gel migration patterns. Unique bands occurred in different treatments, manifesting environmental selection. The long-term field trial showed soil management consistently influenced AOB communities. Significantly, AOB diversity differed at a small scale within a relatively uniform landscape, even in the presence of long-term management practices.

AB - Nitrification is the biological oxidation of NH3 to NO2 − and NO3 − for which ammonia-oxidizing bacteria (AOB) are the functionally dominant group in cropland responsible for the rate-limiting step of NH3 oxidation. Different AOB species have functional differentiation and do not equally contribute to nitrification. This investigation explored if long-term N fertilization and tillage influenced AOB community structure. The study site was a long-term (>40 years) continuous maize (Zea mays L.) experiment with three N fertilization rates (0, 168, and 336 kg ha−1) and either no-tillage (NT) or moldboard plow tillage (PT). We used denaturing gradient gel electrophoresis (DGGE) to analyze PCR-amplified bacterial ammonia monooxygenase genes and detect changes among NH3-oxidizing bacteria. Tillage, fertilization, and sample season all significantly influenced the AOB community. The AOB were more diverse in NT than PT. The AOB became more diverse with increasing N input and were more diverse in summer than winter samples. Nitrosomonas-like and Nitrosospira-like groups were identified based on gel migration patterns. Unique bands occurred in different treatments, manifesting environmental selection. The long-term field trial showed soil management consistently influenced AOB communities. Significantly, AOB diversity differed at a small scale within a relatively uniform landscape, even in the presence of long-term management practices.

KW - Ammonia oxidizing bacteria

KW - Autotrophic nitrification

KW - Community structure

KW - PCR-DGGE

UR - http://www.scopus.com/inward/record.url?scp=85046863364&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85046863364&partnerID=8YFLogxK

U2 - 10.1016/j.apsoil.2018.05.002

DO - 10.1016/j.apsoil.2018.05.002

M3 - Article

AN - SCOPUS:85046863364

SN - 0929-1393

VL - 129

SP - 98

EP - 106

JO - Applied Soil Ecology

JF - Applied Soil Ecology

ER -

Nitrogen, season, and tillage management influence ammonia oxidizing bacterial communities in long-term maize

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this