NMR and MS-based Stable Isotope-Resolved Metabolomics and applications in cancer metabolism

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21 Scopus citations

Abstract

There is considerable interest in defining metabolic reprogramming in human diseases, which is recognized as a hallmark of human cancer. Although radiotracers have a long history in specific metabolic studies, stable isotope-enriched precursors coupled with modern high resolution mass spectrometry and NMR spectroscopy have enabled systematic mapping of metabolic networks and fluxes in cells, tissues and living organisms including humans. These analytical platforms are high in information content, are complementary and cross-validating in terms of compound identification, quantification, and isotope labeling pattern analysis of a large number of metabolites simultaneously. Furthermore, new developments in chemoselective derivatization and in vivo spectroscopy enable tracking of labile/low abundance metabolites and metabolic kinetics in real-time. Here we review developments in Stable Isotope Resolved Metabolomics (SIRM) and recent applications in cancer metabolism using a wide variety of stable isotope tracers that probe both broad and specific aspects of cancer metabolism required for proliferation and survival.

Original languageEnglish
Article number115322
JournalTrAC - Trends in Analytical Chemistry
Volume120
DOIs
StatePublished - Nov 2019

Bibliographical note

Funding Information:
This work was supported in part by National Institutes of health grants: 1P01CA163223-01A1 (to ANL and TWMF), 1U24DK097215-01A1 (to RMH, TWMF, and ANL) and 5R01ES022191-04 (to TWMF and RMH). Some of this text was published as an application note by Cambridge Isotopes Laboratories [MET_RSCH_CANCER (2/13/18)].

Funding Information:
This work was supported in part by National Institutes of health grants: 1P01CA163223-01A1 (to ANL and TWMF ), 1U24DK097215-01A1 (to RMH , TWMF , and ANL ) and 5R01ES022191-04 (to TWMF and RMH ).

Funding Information:
This work was supported in part by National Institutes of health grants: 1P01CA163223-01A1 (to ANL and TWMF), 1U24DK097215-01A1 (to RMH, TWMF, and ANL) and 5R01ES022191-04 (to TWMF and RMH).

Publisher Copyright:
© 2018

Keywords

  • Cancer metabolism
  • Mass spectrometry
  • Model systems
  • NMR
  • SIRM

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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