NMR-based Stable Isotope Resolved Metabolomics in systems biochemistry

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38 Scopus citations

Abstract

Metabolism is the basic activity of live cells, and monitoring the metabolic state provides a dynamic picture of the cells or tissues, and how they respond to external changes, for in disease or treatment with drugs. NMR is an extremely versatile analytical tool that can be applied to a wide range of biochemical problems. Despite its modest sensitivity its versatility make it an ideal tool for analyzing biochemical dynamics both in vitro and in vivo, especially when coupled with its isotope editing capabilities, from which isotope distributions can be readily determined. These are critical for any analyses of flux in live organisms. This review focuses on the utility of NMR spectroscopy in metabolomics, with an emphasis on NMR applications in stable isotope-enriched tracer research for elucidating biochemical pathways and networks with examples from nucleotide biochemistry. The knowledge gained from this area of research provides a ready link to genomic, epigenomic, transcriptomic, and proteomic information to achieve systems biochemical understanding of living cells and organisms.

Original languageEnglish
Pages (from-to)123-131
Number of pages9
JournalArchives of Biochemistry and Biophysics
Volume628
DOIs
StatePublished - Aug 15 2017

Bibliographical note

Funding Information:
This work was supported by the National Institutes of Health [grant numbers 5P01CA163223, U24DK097215, 5R01ES022191].

Publisher Copyright:
© 2017 Elsevier Inc.

Keywords

  • Isotope editing
  • Isotopomer distribution analysis
  • Stable Isotope Resolved Metabolomics (SIRM)

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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