NMR-based Stable Isotope Resolved Metabolomics in systems biochemistry

Andrew N. Lane; Teresa W.M. Fan

doi:10.1016/j.abb.2017.02.009

NMR-based Stable Isotope Resolved Metabolomics in systems biochemistry

Andrew N. Lane, Teresa W.M. Fan

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

Metabolism is the basic activity of live cells, and monitoring the metabolic state provides a dynamic picture of the cells or tissues, and how they respond to external changes, for in disease or treatment with drugs. NMR is an extremely versatile analytical tool that can be applied to a wide range of biochemical problems. Despite its modest sensitivity its versatility make it an ideal tool for analyzing biochemical dynamics both in vitro and in vivo, especially when coupled with its isotope editing capabilities, from which isotope distributions can be readily determined. These are critical for any analyses of flux in live organisms. This review focuses on the utility of NMR spectroscopy in metabolomics, with an emphasis on NMR applications in stable isotope-enriched tracer research for elucidating biochemical pathways and networks with examples from nucleotide biochemistry. The knowledge gained from this area of research provides a ready link to genomic, epigenomic, transcriptomic, and proteomic information to achieve systems biochemical understanding of living cells and organisms.

Original language	English
Pages (from-to)	123-131
Number of pages	9
Journal	Archives of Biochemistry and Biophysics
Volume	628
DOIs	https://doi.org/10.1016/j.abb.2017.02.009
State	Published - Aug 15 2017

Bibliographical note

Publisher Copyright:
© 2017 Elsevier Inc.

Funding

This work was supported by the National Institutes of Health [grant numbers 5P01CA163223, U24DK097215, 5R01ES022191].

Funders	Funder number
National Institutes of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases	U24DK097215
U.S. Department of Energy Chinese Academy of Sciences Guangzhou Municipal Science and Technology Project Oak Ridge National Laboratory Extreme Science and Engineering Discovery Environment National Science Foundation National Energy Research Scientific Computing Center National Natural Science Foundation of China	0447479
National Childhood Cancer Registry – National Cancer Institute	P30CA177558, P01CA163223
National Institutes of Health/National Institute of Environmental Health Sciences	R01ES022191

Keywords

Isotope editing
Isotopomer distribution analysis
Stable Isotope Resolved Metabolomics (SIRM)

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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10.1016/j.abb.2017.02.009

Cite this

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abstract = "Metabolism is the basic activity of live cells, and monitoring the metabolic state provides a dynamic picture of the cells or tissues, and how they respond to external changes, for in disease or treatment with drugs. NMR is an extremely versatile analytical tool that can be applied to a wide range of biochemical problems. Despite its modest sensitivity its versatility make it an ideal tool for analyzing biochemical dynamics both in vitro and in vivo, especially when coupled with its isotope editing capabilities, from which isotope distributions can be readily determined. These are critical for any analyses of flux in live organisms. This review focuses on the utility of NMR spectroscopy in metabolomics, with an emphasis on NMR applications in stable isotope-enriched tracer research for elucidating biochemical pathways and networks with examples from nucleotide biochemistry. The knowledge gained from this area of research provides a ready link to genomic, epigenomic, transcriptomic, and proteomic information to achieve systems biochemical understanding of living cells and organisms.",

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AB - Metabolism is the basic activity of live cells, and monitoring the metabolic state provides a dynamic picture of the cells or tissues, and how they respond to external changes, for in disease or treatment with drugs. NMR is an extremely versatile analytical tool that can be applied to a wide range of biochemical problems. Despite its modest sensitivity its versatility make it an ideal tool for analyzing biochemical dynamics both in vitro and in vivo, especially when coupled with its isotope editing capabilities, from which isotope distributions can be readily determined. These are critical for any analyses of flux in live organisms. This review focuses on the utility of NMR spectroscopy in metabolomics, with an emphasis on NMR applications in stable isotope-enriched tracer research for elucidating biochemical pathways and networks with examples from nucleotide biochemistry. The knowledge gained from this area of research provides a ready link to genomic, epigenomic, transcriptomic, and proteomic information to achieve systems biochemical understanding of living cells and organisms.

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NMR-based Stable Isotope Resolved Metabolomics in systems biochemistry

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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