Abstract
Marine natural products have become an increasingly important source of new drug leads during recent years. In an attempt to identify novel anti-microbial natural products by bioprospecting deep-sea Actinobacteria, three new angucyclines, nocardiopsistins A-C, were isolated from Nocardiopsis sp. strain HB-J378. Notably, the supplementation of the rare earth salt Lanthanum chloride (LaCl3) during fermentation of HB-J378 significantly increased the yield of these angucyclines. The structures of nocardiopsistins A-C were identified by 1D and 2D NMR and HR-MS data. Nocardiopsistins A-C have activity against MRSA (methicillin-resistant Staphylococcus aureus) with MICs of 3.12–12.5 μg/mL; the potency of nocardiopsistin B is similar to that of the positive control, chloramphenicol. Bioinformatic analysis of the draft genome of HB-J378 identified a set of three core genes in a biosynthetic gene cluster that encode a typical aromatic or type II polyketide synthase (PKS) system, including ketoacyl:ACP synthase α-subunit (KSα), β-subunit (KSβ) and acyl carrier protein (ACP). The production of nocardiopsistins A-C was abolished when the three genes were knocked out, indicating their indispensable role in the production of nocardiopsistins.
Original language | English |
---|---|
Pages (from-to) | 246-251 |
Number of pages | 6 |
Journal | Synthetic and Systems Biotechnology |
Volume | 3 |
Issue number | 4 |
DOIs | |
State | Published - Dec 2018 |
Bibliographical note
Publisher Copyright:© 2018
Keywords
- Actinobacteria
- Angucycline
- Anti-MRSA
- LaCl
- Nocardiopsis
- Nocardiopsistins
ASJC Scopus subject areas
- Structural Biology
- Biomedical Engineering
- Applied Microbiology and Biotechnology
- Genetics