Abstract
Culture conductivity and on-line NADH fluorescence were used to measure cellular growth in plant cell suspension cultures of Podophyllum hexandrum. An inverse correlation between dry cell weight and medium conductivity was observed during shake flask cultivation. A linear relationship between dry cell weight and culture NADH fluorescence was obtained during the exponential phase of batch cultivation in a bioreactor under the pH stat (pH 6) conditions. It was observed that conductivity measurement were suitable for biomass characterisation under highly dynamic uncontrolled shake flask cultivation conditions. However, if the acid/alkali feeding is done for pH control the conductivity measurement could not be applied. On the other hand the NADH fluorescence measurement allowed online-in situ biomass monitoring of rather heteroge-nous plant cell suspension cultures in bioreactor even under the most desirable pH stat conditions.
| Original language | English |
|---|---|
| Pages (from-to) | 331-334 |
| Number of pages | 4 |
| Journal | Biotechnology and Bioprocess Engineering |
| Volume | 7 |
| Issue number | 6 |
| DOIs | |
| State | Published - 2002 |
Keywords
- Conductivity measurement
- NADH measurement
- On-line biomass measurement
- Podophyllum hexandrum
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
- Biomedical Engineering