Non-surgical therapy reduces presence of JP2 clone in localized aggressive periodontitis

Danielle K. Burgess, Hong Huang, Peter Harrison, Theodora Kompotiati, Ikramuddin Aukhil, Luciana M. Shaddox

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Background: Previous studies have provided substantial evidence of the association of Aggregatibacter actinomycetemcomitans, and its highly leukotoxic JP2 genotype, with localized aggressive periodontitis (LAgP). The present study aims to evaluate presence of JP2 in individuals with LAgP after periodontal treatment. Methods: Sixty African-American patients with LAgP, aged 5 to 25 years, were examined. At baseline, probing depth (PD), clinical attachment level (CAL), bleeding on probing, and plaque index were measured, and subgingival plaque was collected from LAgP diseased and healthy sites for each participant. Patients received whole-mouth ultrasonic debridement, scaling and root planing, and a 7-day prescription of amoxicillin and metronidazole. Participants were reevaluated and resampled and received regular maintenance therapy at 3, 6, and 12 months after treatment. Polymerase chain reaction was used to detect presence of the JP2 genotype before and after treatment. Results: At baseline, the JP2 sequence was identified in 75% of LAgP diseased sites and in 56.67% of healthy sites. At 3, 6, and 12 months after treatment, the number of patients was 40, 31, and 31, respectively, and JP2 detection decreased to 17.5%, 6.45%, and 3.23%, respectively, in diseased sites (P <0.001) and to 2.5%, 3.23%, and 0%, respectively, in healthy sites (P <0.001). Clinical parameters of disease were also significantly reduced after therapy (P <0.001). Additionally, significant correlations were observed between JP2 presence and mean PD (P <0.002) and CAL (P <0.001), after therapy. Conclusion: Periodontal therapy was successful in reducing clinical parameters of LAgP and subgingival presence of JP2 in diseased and healthy sites.

Original languageEnglish
Pages (from-to)1263-1270
Number of pages8
JournalJournal of Periodontology
Volume88
Issue number12
DOIs
StatePublished - Dec 2017

Bibliographical note

Funding Information:
The authors thank the National Institutes of Health/ National Institute of Dental and Craniofacial Research, Bethesda, Maryland (R01DE019456), for the financial support awarded to this research (PI: Luciana M. Shaddox), as well as the doctors and staff at dental centers at the Leon County Health Department, Tallahassee, Florida; Duval County Health Department, Jacksonville, Florida; and Jackson County Health Department, Marianna, Florida for assistance in coordinating visits to their clinics, patient referrals, and dental care. This study is registered in Clinicaltrials.gov (#NCT01330719). The authors report no conflicts of interest related to this study.

Keywords

  • Aggregatibacter actinomycetemcomitans
  • Bacteria
  • Inflammation
  • Periodontitis
  • Therapeutics
  • Virulence

ASJC Scopus subject areas

  • Periodontics

Fingerprint

Dive into the research topics of 'Non-surgical therapy reduces presence of JP2 clone in localized aggressive periodontitis'. Together they form a unique fingerprint.

Cite this