A noncontact electron multiplying charge-coupled-device (EMCCD)-based speckle contrast diffuse correlation tomography (scDCT) technology has been recently developed in our laboratory, allowing for noninvasive three-dimensional measurement of tissue blood flow distributions. One major remaining constraint in the scDCT is the assumption of a semi-infinite tissue volume with a flat surface, which affects the image reconstruction accuracy for tissues with irregular geometries. An advanced photometric stereo technique (PST) was integrated into the scDCT system to obtain the surface geometry in real time for image reconstruction. Computer simulations demonstrated that a priori knowledge of tissue surface geometry is crucial for precisely reconstructing the anomaly with blood flow contrast. Importantly, the innovative integration design with one single- EMCCD camera for both PST and scDCT data collection obviates the need for offline alignment of sources and detectors on the tissue boundary. The in vivo imaging capability of the updated scDCT is demonstrated by imaging dynamic changes in forearm blood flow distribution during a cuff-occlusion procedure. The feasibility and safety in clinical use are evidenced by intraoperative imaging of mastectomy skin flaps and comparison with fluorescence angiography.
|Journal||Journal of Biomedical Optics|
|State||Published - Sep 1 2018|
Bibliographical noteFunding Information:
We acknowledge support from the National Institutes of Health Nos. R01-CA149274 (G. Y.), R21-AR062356 (G. Y.), and R21-HD091118 (G. Y.), American Heart Association Grant-In-Aid 16GRNT30820006 (G. Y.); National Endowment for Plastic Surgery, Plastic Surgery Foundation No. 3048112770 (L. W. and G. Y.); National Science Foundation EPSCoR #1539068 (G. Y.); and the Halcomb Fellowship in Medicine and Engineering at the University of the Kentucky (S. M.).
© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE).
- blood flow distribution
- diffuse optical imaging
- mastectomy skin flap necrosis.
- tissue surface geometry
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Biomedical Engineering