Nonculturability Might Underestimate the Occurrence of Campylobacter in Broiler Litter

Issmat I. Kassem, Yosra A. Helmy, Dipak Kathayat, Rosario A. Candelero-Rueda, Anand Kumar, Loic Deblais, Huang Chi Huang, Orhan Sahin, Qijing Zhang, Gireesh Rajashekara

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

We investigated the contribution of litter to the occurrence of Campylobacter on three broiler farms, which were known to have low (LO) and high (HI-A and HI-B) Campylobacter prevalence. For this purpose, we collected litter samples (n = 288) during and after two rearing cycles from each farm. We evaluated the occurrence of Campylobacter (using selective enrichment and quantitative real-time polymerase chain reaction [q-PCR] analysis) in the litter samples as well as the litter's pH and moisture content. Ceca from each flock (n = 144) were harvested at slaughter age and used to quantify Campylobacter colony-forming units (CFUs). Campylobacter was only retrieved from 7 litter samples that were collected from HI-A and HI-B during the growing period, but no Campylobacter was isolated from LO farms. The q-PCR analysis detected Campylobacter in pooled litter samples from all three farms. However, in litter collected during the same rotation, Campylobacter levels were significantly higher (p < 0.05) in HI-A and HI-B litter samples in comparison to those in LO. Cecal samples from HI-A and HI-B yielded relatively high numbers of Campylobacter CFUs, which were undetectable in LO samples. Litter's pH and moisture did not affect the overall occurrence of Campylobacter in litter and ceca on any of the farms. Our data suggest that Campylobacter was generally more abundant in litter that was collected from farms with highly colonized flocks. Therefore, better approaches for assessing the occurrence of Campylobacter in litter might be warranted in order to reduce the dissemination of these pathogens on and off poultry farms.

Original languageEnglish
Pages (from-to)472-477
Number of pages6
JournalFoodborne Pathogens and Disease
Volume14
Issue number8
DOIs
StatePublished - Aug 2017

Bibliographical note

Funding Information:
Research in the Rajashekara laboratory is supported by funds from the Agriculture and Food Research Initiative (AFRI, U.S. Department of Agriculture, Grant No. 2012-68003-19679), the Ohio Agricultural Research and Development Center, and the Ohio State University.

Publisher Copyright:
Copyright © 2017 Mary Ann Liebert, Inc.

Keywords

  • broilers
  • Campylobacter
  • chickens
  • control
  • food safety
  • litter
  • moisture
  • pH

ASJC Scopus subject areas

  • Microbiology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Animal Science and Zoology

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