Nondestructive DNA Extraction Techniques for Soybean (Glycine Max) Seeds

Maythem AL-Amery, Hirotada Fukushige, William Serson, David Hildebrand

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


While maintaining high germination rates, genotyping single seeds are useful in breeding and genetics. Nondestructive single-seed genomic DNA extraction protocols using 12 mg cotyledon tissue with a modified cetyl trimethyl ammonium bromide (CTAB) technique and a commercial seed DNA extraction kit using 1 mg cotyledon tissue were developed for dry soybean seeds and cross-verified with leaf DNA analysis. The DNA extracted by these methods was sufficient for polymerase chain reaction (PCR), and the results were reproducible for several endogenous genes and transgenes in D-myo-inositol 3-phosphate synthase (MIPS) mutant seeds (GM-lpa-TW1). This single-seed DNA-analysis method is amenable to rapid high-throughput genotype screening and verifying the genetic purity of seed stocks.

Original languageEnglish
Pages (from-to)165-175
Number of pages11
JournalJournal of Crop Improvement
Issue number2
StatePublished - Mar 3 2016

Bibliographical note

Publisher Copyright:
© 2016 Taylor & Francis.


  • CTAB
  • DNA extraction
  • high throughput genotyping
  • molecular breeding
  • seed chips

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Soil Science
  • Plant Science


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