Novel Hypocholesterolemic Peptides Derived from Silver Carp Muscle: The Modulatory Effects on Enterohepatic Cholesterol Metabolism In Vitro and In Vivo

Kai Wang; Lihua Han; Yuqing Tan; Hui Hong; Hongbing Fan; Yongkang Luo

doi:10.1021/acs.jafc.2c09092

Novel Hypocholesterolemic Peptides Derived from Silver Carp Muscle: The Modulatory Effects on Enterohepatic Cholesterol Metabolism In Vitro and In Vivo

Kai Wang, Lihua Han, Yuqing Tan, Hui Hong, Hongbing Fan, Yongkang Luo

Research output: Contribution to journal › Article › peer-review

Abstract

This research aimed to investigate the effect of silver carp hydrolysates (SCHs) on hypercholesterolemia and enterohepatic cholesterol metabolism. Results showed that in vitro gastrointestinal digestion products of Alcalase-SCH (GID-Alcalase) exhibited the highest inhibitory activity of cholesterol absorption mainly through downregulating the expression of essential genes related to cholesterol transport in a Caco-2 monolayer. After being absorbed by the Caco-2 monolayer, GID-Alcalase increased the low-density lipoprotein (LDL) uptake in HepG2 cells by enhancing the protein level of the LDL receptor (LDLR). The in vivo experiment showed that long-term intervention of Alcalase-SCH ameliorated hypercholesterolemia in ApoE^-/- mice fed with a Western diet (WD). After transepithelial transport, four novel peptides (TKY, LIL, FPK, and IAIM) were identified, and these peptides possessed dual hypocholesterolemic functions including inhibition of cholesterol absorption and promotion of peripheric LDL uptake. Our results indicated for the first time the potential of SCHs as functional food ingredients for the management of hypercholesterolemia.

Original language	English
Pages (from-to)	5565-5575
Number of pages	11
Journal	Journal of Agricultural and Food Chemistry
Volume	71
Issue number	14
DOIs	https://doi.org/10.1021/acs.jafc.2c09092
State	Published - Apr 12 2023

Bibliographical note

Funding Information:
This study was supported by the China Agriculture Research System (CARS-45) and the National Science and Technology Ministry of China (Award number 2017YFD0400200).

Publisher Copyright:
© 2023 American Chemical Society.

Keywords

cholesterol absorption
hypercholesterolemia mice
LDL uptake
peptide sequences
silver carp muscle hydrolysate
transepithelial transport

ASJC Scopus subject areas

Chemistry (all)
Agricultural and Biological Sciences (all)

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10.1021/acs.jafc.2c09092

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title = "Novel Hypocholesterolemic Peptides Derived from Silver Carp Muscle: The Modulatory Effects on Enterohepatic Cholesterol Metabolism In Vitro and In Vivo",

abstract = "This research aimed to investigate the effect of silver carp hydrolysates (SCHs) on hypercholesterolemia and enterohepatic cholesterol metabolism. Results showed that in vitro gastrointestinal digestion products of Alcalase-SCH (GID-Alcalase) exhibited the highest inhibitory activity of cholesterol absorption mainly through downregulating the expression of essential genes related to cholesterol transport in a Caco-2 monolayer. After being absorbed by the Caco-2 monolayer, GID-Alcalase increased the low-density lipoprotein (LDL) uptake in HepG2 cells by enhancing the protein level of the LDL receptor (LDLR). The in vivo experiment showed that long-term intervention of Alcalase-SCH ameliorated hypercholesterolemia in ApoE-/- mice fed with a Western diet (WD). After transepithelial transport, four novel peptides (TKY, LIL, FPK, and IAIM) were identified, and these peptides possessed dual hypocholesterolemic functions including inhibition of cholesterol absorption and promotion of peripheric LDL uptake. Our results indicated for the first time the potential of SCHs as functional food ingredients for the management of hypercholesterolemia.",

keywords = "cholesterol absorption, hypercholesterolemia mice, LDL uptake, peptide sequences, silver carp muscle hydrolysate, transepithelial transport",

author = "Kai Wang and Lihua Han and Yuqing Tan and Hui Hong and Hongbing Fan and Yongkang Luo",

note = "Funding Information: This study was supported by the China Agriculture Research System (CARS-45) and the National Science and Technology Ministry of China (Award number 2017YFD0400200). Publisher Copyright: {\textcopyright} 2023 American Chemical Society.",

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AU - Tan, Yuqing

AU - Hong, Hui

AU - Fan, Hongbing

AU - Luo, Yongkang

N1 - Funding Information: This study was supported by the China Agriculture Research System (CARS-45) and the National Science and Technology Ministry of China (Award number 2017YFD0400200). Publisher Copyright: © 2023 American Chemical Society.

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N2 - This research aimed to investigate the effect of silver carp hydrolysates (SCHs) on hypercholesterolemia and enterohepatic cholesterol metabolism. Results showed that in vitro gastrointestinal digestion products of Alcalase-SCH (GID-Alcalase) exhibited the highest inhibitory activity of cholesterol absorption mainly through downregulating the expression of essential genes related to cholesterol transport in a Caco-2 monolayer. After being absorbed by the Caco-2 monolayer, GID-Alcalase increased the low-density lipoprotein (LDL) uptake in HepG2 cells by enhancing the protein level of the LDL receptor (LDLR). The in vivo experiment showed that long-term intervention of Alcalase-SCH ameliorated hypercholesterolemia in ApoE-/- mice fed with a Western diet (WD). After transepithelial transport, four novel peptides (TKY, LIL, FPK, and IAIM) were identified, and these peptides possessed dual hypocholesterolemic functions including inhibition of cholesterol absorption and promotion of peripheric LDL uptake. Our results indicated for the first time the potential of SCHs as functional food ingredients for the management of hypercholesterolemia.

AB - This research aimed to investigate the effect of silver carp hydrolysates (SCHs) on hypercholesterolemia and enterohepatic cholesterol metabolism. Results showed that in vitro gastrointestinal digestion products of Alcalase-SCH (GID-Alcalase) exhibited the highest inhibitory activity of cholesterol absorption mainly through downregulating the expression of essential genes related to cholesterol transport in a Caco-2 monolayer. After being absorbed by the Caco-2 monolayer, GID-Alcalase increased the low-density lipoprotein (LDL) uptake in HepG2 cells by enhancing the protein level of the LDL receptor (LDLR). The in vivo experiment showed that long-term intervention of Alcalase-SCH ameliorated hypercholesterolemia in ApoE-/- mice fed with a Western diet (WD). After transepithelial transport, four novel peptides (TKY, LIL, FPK, and IAIM) were identified, and these peptides possessed dual hypocholesterolemic functions including inhibition of cholesterol absorption and promotion of peripheric LDL uptake. Our results indicated for the first time the potential of SCHs as functional food ingredients for the management of hypercholesterolemia.

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Novel Hypocholesterolemic Peptides Derived from Silver Carp Muscle: The Modulatory Effects on Enterohepatic Cholesterol Metabolism In Vitro and In Vivo

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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