This research aimed to investigate the effect of silver carp hydrolysates (SCHs) on hypercholesterolemia and enterohepatic cholesterol metabolism. Results showed that in vitro gastrointestinal digestion products of Alcalase-SCH (GID-Alcalase) exhibited the highest inhibitory activity of cholesterol absorption mainly through downregulating the expression of essential genes related to cholesterol transport in a Caco-2 monolayer. After being absorbed by the Caco-2 monolayer, GID-Alcalase increased the low-density lipoprotein (LDL) uptake in HepG2 cells by enhancing the protein level of the LDL receptor (LDLR). The in vivo experiment showed that long-term intervention of Alcalase-SCH ameliorated hypercholesterolemia in ApoE-/- mice fed with a Western diet (WD). After transepithelial transport, four novel peptides (TKY, LIL, FPK, and IAIM) were identified, and these peptides possessed dual hypocholesterolemic functions including inhibition of cholesterol absorption and promotion of peripheric LDL uptake. Our results indicated for the first time the potential of SCHs as functional food ingredients for the management of hypercholesterolemia.
|Number of pages||11|
|Journal||Journal of Agricultural and Food Chemistry|
|State||Published - Apr 12 2023|
Bibliographical noteFunding Information:
This study was supported by the China Agriculture Research System (CARS-45) and the National Science and Technology Ministry of China (Award number 2017YFD0400200).
© 2023 American Chemical Society.
- cholesterol absorption
- hypercholesterolemia mice
- LDL uptake
- peptide sequences
- silver carp muscle hydrolysate
- transepithelial transport
ASJC Scopus subject areas
- Chemistry (all)
- Agricultural and Biological Sciences (all)