Nuclear and mitochondrial genomic resources for the meltwater stonefly (Plecoptera: Nemouridae), Lednia tumana (Ricker, 1952)

Scott Hotaling; Joanna L. Kelley; David W. Weisrock

doi:10.1080/01650424.2019.1639764

Nuclear and mitochondrial genomic resources for the meltwater stonefly (Plecoptera: Nemouridae), Lednia tumana (Ricker, 1952)

Scott Hotaling, Joanna L. Kelley, David W. Weisrock

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

With more than 3,700 described species, stoneflies (Order Plecoptera) are an important component of global aquatic biodiversity. The meltwater stonefly Lednia tumana (Ricker, 1952) is endemic to alpine streams of Glacier National Park and has been petitioned for listing under the U.S. Endangered Species Act (ESA) due to climate change-induced loss of alpine glaciers and snowfields. Here, we present de novo assemblies of the nuclear (∼520 million base pairs [bp]) and mitochondrial (15,014-bp) genomes for L. tumana. The L. tumana (Family Nemouridae) nuclear genome is the most complete stonefly genome reported to date, with ∼71% of genes present in complete form and more than 4,600 contigs longer than 10-kilobases (kb). The L. tumana mitochondrial genome is the second for the family Nemouridae and the first from North America. Together, both genomes represent important foundational resources, setting the stage for future efforts to understand the evolution of L. tumana, stoneflies, and aquatic insects worldwide.

Original language	English
Pages (from-to)	362-369
Number of pages	8
Journal	Aquatic Insects
Volume	40
Issue number	4
DOIs	https://doi.org/10.1080/01650424.2019.1639764
State	Published - Oct 2 2019

Bibliographical note

Publisher Copyright:
© 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.

Funding

This research was supported by the University of Kentucky (UK) and National Science Foundation (DEB-0949532). We thank Alan Lemmon and Emily Lemmon for advice and assistance with sequencing. Computational resources were provided by the UK Center for Computational Sciences and the Lipscomb High Performance Computing Cluster, as well as the Washington State University Center for Institutional Research Computing.

Funders	Funder number
UK Center for Computational Sciences
National Science Foundation (NSF)	DEB-0949532
University of Kentucky
State of Washington Water Research Center, Washington State University

Keywords

Lednia
Nemouridae
Plecoptera
USA
genomics
nuclear genome

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Aquatic Science
Insect Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1080/01650424.2019.1639764

Cite this

@article{09bfa635223849acbd180032959d2766,

title = "Nuclear and mitochondrial genomic resources for the meltwater stonefly (Plecoptera: Nemouridae), Lednia tumana (Ricker, 1952)",

abstract = "With more than 3,700 described species, stoneflies (Order Plecoptera) are an important component of global aquatic biodiversity. The meltwater stonefly Lednia tumana (Ricker, 1952) is endemic to alpine streams of Glacier National Park and has been petitioned for listing under the U.S. Endangered Species Act (ESA) due to climate change-induced loss of alpine glaciers and snowfields. Here, we present de novo assemblies of the nuclear (∼520 million base pairs [bp]) and mitochondrial (15,014-bp) genomes for L. tumana. The L. tumana (Family Nemouridae) nuclear genome is the most complete stonefly genome reported to date, with ∼71\% of genes present in complete form and more than 4,600 contigs longer than 10-kilobases (kb). The L. tumana mitochondrial genome is the second for the family Nemouridae and the first from North America. Together, both genomes represent important foundational resources, setting the stage for future efforts to understand the evolution of L. tumana, stoneflies, and aquatic insects worldwide.",

keywords = "Lednia, Nemouridae, Plecoptera, USA, genomics, nuclear genome",

author = "Scott Hotaling and Kelley, \{Joanna L.\} and Weisrock, \{David W.\}",

note = "Publisher Copyright: {\textcopyright} 2019, {\textcopyright} 2019 Informa UK Limited, trading as Taylor \& Francis Group.",

year = "2019",

month = oct,

day = "2",

doi = "10.1080/01650424.2019.1639764",

language = "English",

volume = "40",

pages = "362--369",

number = "4",

}

TY - JOUR

T1 - Nuclear and mitochondrial genomic resources for the meltwater stonefly (Plecoptera: Nemouridae), Lednia tumana (Ricker, 1952)

AU - Hotaling, Scott

AU - Kelley, Joanna L.

AU - Weisrock, David W.

PY - 2019/10/2

Y1 - 2019/10/2

N2 - With more than 3,700 described species, stoneflies (Order Plecoptera) are an important component of global aquatic biodiversity. The meltwater stonefly Lednia tumana (Ricker, 1952) is endemic to alpine streams of Glacier National Park and has been petitioned for listing under the U.S. Endangered Species Act (ESA) due to climate change-induced loss of alpine glaciers and snowfields. Here, we present de novo assemblies of the nuclear (∼520 million base pairs [bp]) and mitochondrial (15,014-bp) genomes for L. tumana. The L. tumana (Family Nemouridae) nuclear genome is the most complete stonefly genome reported to date, with ∼71% of genes present in complete form and more than 4,600 contigs longer than 10-kilobases (kb). The L. tumana mitochondrial genome is the second for the family Nemouridae and the first from North America. Together, both genomes represent important foundational resources, setting the stage for future efforts to understand the evolution of L. tumana, stoneflies, and aquatic insects worldwide.

AB - With more than 3,700 described species, stoneflies (Order Plecoptera) are an important component of global aquatic biodiversity. The meltwater stonefly Lednia tumana (Ricker, 1952) is endemic to alpine streams of Glacier National Park and has been petitioned for listing under the U.S. Endangered Species Act (ESA) due to climate change-induced loss of alpine glaciers and snowfields. Here, we present de novo assemblies of the nuclear (∼520 million base pairs [bp]) and mitochondrial (15,014-bp) genomes for L. tumana. The L. tumana (Family Nemouridae) nuclear genome is the most complete stonefly genome reported to date, with ∼71% of genes present in complete form and more than 4,600 contigs longer than 10-kilobases (kb). The L. tumana mitochondrial genome is the second for the family Nemouridae and the first from North America. Together, both genomes represent important foundational resources, setting the stage for future efforts to understand the evolution of L. tumana, stoneflies, and aquatic insects worldwide.

KW - Lednia

KW - Nemouridae

KW - Plecoptera

KW - USA

KW - genomics

KW - nuclear genome

UR - http://www.scopus.com/inward/record.url?scp=85070920025&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85070920025&partnerID=8YFLogxK

U2 - 10.1080/01650424.2019.1639764

DO - 10.1080/01650424.2019.1639764

M3 - Article

AN - SCOPUS:85070920025

SN - 0165-0424

VL - 40

SP - 362

EP - 369

JO - Aquatic Insects

JF - Aquatic Insects

IS - 4

ER -

Nuclear and mitochondrial genomic resources for the meltwater stonefly (Plecoptera: Nemouridae), Lednia tumana (Ricker, 1952)

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this