Nucleolin/Nsr1p binds to the 3′ noncoding region of the tombusvirus RNA and inhibits replication

Yi Jiang; Zhenghe Li; Peter D. Nagy

doi:10.1016/j.virol.2009.10.007

Nucleolin/Nsr1p binds to the 3′ noncoding region of the tombusvirus RNA and inhibits replication

Yi Jiang, Zhenghe Li, Peter D. Nagy

Plant Pathology

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Previous genome-wide screens identified > 100 host genes affecting tombusvirus replication using yeast model host. One of those factors was Nsr1p (nucleolin), which is an abundant RNA-binding shuttle protein involved in rRNA maturation and ribosome assembly. We find that overexpression of Nsr1p in yeast or in Nicotiana benthamiana inhibited the accumulation of tombusvirus RNA by ∼10-fold. Regulated overexpression of Nsr1p revealed that Nsr1p should be present at the beginning of viral replication for efficient inhibition, suggesting that Nsr1p inhibits an early step in the replication process. In vitro experiments revealed that Nsr1p binds preferably to the 3′ UTR in the viral RNA. The purified recombinant Nsr1p inhibited the in vitro replication of the viral RNA in a yeast cell-free assay when preincubated with the viral RNA before the assay. These data support the model that Nsr1p/nucleolin inhibits tombusvirus replication by interfering with the recruitment of the viral RNA for replication.

Original language	English
Pages (from-to)	10-20
Number of pages	11
Journal	Virology
Volume	396
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2009.10.007
State	Published - Jan 5 2010

Bibliographical note

Funding Information:
The authors thank Drs. Judit Pogany and Daniel Barajas. The authors are grateful to Dr. K. Nakamura for sharing plasmid pGWB5 and to Dr. M. Goodin for the At-Fibrillarin1-RFP transgenic N. benthamiana and anti-GFP and anti-chicken antibodies. This work was supported by the National Science Foundation (IOB-0517218), NIH-NIAID 5R21AI072170-02, and the Kentucky Tobacco Research and Development Center.

Keywords

Host factor
Nucleolin, Nicotiana benthamiana
Tomato bushy stunt virus
Yeast
nsr1

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2009.10.007

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title = "Nucleolin/Nsr1p binds to the 3′ noncoding region of the tombusvirus RNA and inhibits replication",

abstract = "Previous genome-wide screens identified > 100 host genes affecting tombusvirus replication using yeast model host. One of those factors was Nsr1p (nucleolin), which is an abundant RNA-binding shuttle protein involved in rRNA maturation and ribosome assembly. We find that overexpression of Nsr1p in yeast or in Nicotiana benthamiana inhibited the accumulation of tombusvirus RNA by ∼10-fold. Regulated overexpression of Nsr1p revealed that Nsr1p should be present at the beginning of viral replication for efficient inhibition, suggesting that Nsr1p inhibits an early step in the replication process. In vitro experiments revealed that Nsr1p binds preferably to the 3′ UTR in the viral RNA. The purified recombinant Nsr1p inhibited the in vitro replication of the viral RNA in a yeast cell-free assay when preincubated with the viral RNA before the assay. These data support the model that Nsr1p/nucleolin inhibits tombusvirus replication by interfering with the recruitment of the viral RNA for replication.",

keywords = "Host factor, Nucleolin, Nicotiana benthamiana, Tomato bushy stunt virus, Yeast, nsr1",

author = "Yi Jiang and Zhenghe Li and Nagy, {Peter D.}",

note = "Funding Information: The authors thank Drs. Judit Pogany and Daniel Barajas. The authors are grateful to Dr. K. Nakamura for sharing plasmid pGWB5 and to Dr. M. Goodin for the At-Fibrillarin1-RFP transgenic N. benthamiana and anti-GFP and anti-chicken antibodies. This work was supported by the National Science Foundation (IOB-0517218), NIH-NIAID 5R21AI072170-02, and the Kentucky Tobacco Research and Development Center. ",

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AU - Li, Zhenghe

AU - Nagy, Peter D.

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N2 - Previous genome-wide screens identified > 100 host genes affecting tombusvirus replication using yeast model host. One of those factors was Nsr1p (nucleolin), which is an abundant RNA-binding shuttle protein involved in rRNA maturation and ribosome assembly. We find that overexpression of Nsr1p in yeast or in Nicotiana benthamiana inhibited the accumulation of tombusvirus RNA by ∼10-fold. Regulated overexpression of Nsr1p revealed that Nsr1p should be present at the beginning of viral replication for efficient inhibition, suggesting that Nsr1p inhibits an early step in the replication process. In vitro experiments revealed that Nsr1p binds preferably to the 3′ UTR in the viral RNA. The purified recombinant Nsr1p inhibited the in vitro replication of the viral RNA in a yeast cell-free assay when preincubated with the viral RNA before the assay. These data support the model that Nsr1p/nucleolin inhibits tombusvirus replication by interfering with the recruitment of the viral RNA for replication.

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Nucleolin/Nsr1p binds to the 3′ noncoding region of the tombusvirus RNA and inhibits replication

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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