Nucleopolyhedrovirus pathology in spruce budworm larvae

J. W. Barrett, T. R. Ladd, M. J. Primavera, A. Retnakaran, S. S. Sohi, S. R. Palli

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Choristoneura fumiferana (Clemens) (Lepidoptera: Tortricidae) multiple nucleopolyhedrovirus (CfMNPV) expressing green fluorescent protein was used to study aspects of nucleopolyhedrovirus infection in the spruce budworm. The temporal and spatial distribution of fluorescence indicated that the virus infected the midgut, entered the tracheal system, and traveled to the epidermis, fat body, and muscles. In contrast to Autographa californica (Speyer) (Lepidoptera: Noctuidae) multiple nucleopolyhedrovirus (AcMNPV) infection, hemocytes from infected C. fumiferana did not exhibit fluorescence until after CfMNPV had passed from the midgut into the tracheae. Therefore the role of hemocytes may be limited during CfMNPV infection. Also the fluorescence pattern spread from the tracheolar cells to tracheal epithelial cells throughout the tracheal system. Our results indicate that the temporal and spatial events involved in CfMNPV infection of C. fumiferana larvae are consistent with those observed in other lepidopteran hosts infected with AcMNPV. Minor deviations between these two systems may be attributed to differences in virulence, infection rate, and possibly host range of the virus.

Original languageEnglish
Pages (from-to)581-590
Number of pages10
JournalCanadian Entomologist
Volume132
Issue number5
DOIs
StatePublished - 2000

Bibliographical note

Funding Information:
The research was supported by the Canadian Forest Service.and the National Biotechnology Strategy Fund grant to SRP. We thank Basil Arif and Hilary Lauzon for supplying C ' N P V plasmid library and Karen Jamieson for editorial help.

ASJC Scopus subject areas

  • Structural Biology
  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Molecular Biology
  • Insect Science

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