Nucleotide sequence changes produced by mutations in the lac promoter of Escherichia coli

Robert C. Dickson, John Abelson, Peter Johnson, William S. Reznikoff, Wayne M. Barnes

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39 Scopus citations


Nucleotide changes occurring in class I mutants of the lac promoter have been determined. Mutants L8, L37, L65 and L592 produce a G·C to T·A transition at nucleotide 19, while L29, L146 and L614 produce a C·G to A·T transition at nucleotide 28. These two mutational sites are symmetrically located in a sequence in which 12 of 14 nucleotides are related by a dyad axis. This region may be the binding site for the catabolite gene activator protein. In a previous publication (Dickson et al., 1975)_we presented the nucleotide changes seen in the class II promoter mutants, L241 and L305, and the class III promoter mutant, pr1a. Here we present the data which determined these nucleotide changes.

Original languageEnglish
Pages (from-to)65-75
Number of pages11
JournalJournal of Molecular Biology
Issue number1
StatePublished - Mar 25 1977

Bibliographical note

Funding Information:
This work was supported by National Institutes of Health (NIH) grants 1-1%01-GM-19670 and CA 10984, by National Science Foundation grant GB-20462, by the Cancer Research Coordinating Committee of the University of California, and by the Wisconsin Alumni Research Foundation. One of us (J. A.) was supported by a faculty research award from the American Cancer Society. Another author (W. S. 1%.) was supported by a career development award S-K04-GM-30970 from NIH, and a third author (W. M. B.) was supported by NIH training grant GM-00236.

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology


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