TY - JOUR
T1 - O-phenylenediamine-modified fiber electrodes for the detection of nitric oxide
AU - Friedemann, Marilyn N.
AU - Robinson, Scott W.
AU - Gerhardt, Greg A.
PY - 1996/8/1
Y1 - 1996/8/1
N2 - Nitric oxide (NO.) sensors were prepared using o-phenylenediamine (o-PD) and Nafion to modify the surface of 30 μm diameter carbon fiber electrodes. These electrodes were compared with nickel porphyrin-type NO. sensors that have already been described. High-speed chronoamperometry, amperometry, and differential pulse voltammetty were used to compare the performance of sensors modified with various combinations of Nafion, o-PD, or nickel(II) meso-tetrakis(3-methoxy-4-hydroxyphenyl)porphyrin (Ni-TMPP), in order to determine which electrodes had the most sensitivity and selectivity for NO.. Our findings showed that electrodes treated with Nafion first, followed by o-PD, were very sensitive to NO., with a detection limit of 35 ± 7 nM. In addition, o-PD electrodes were also very selective against ascorbate (>600:1), dopamine (>300:1), and nitrite (>900:1). Moreover, in the range of 0-6 μM NO., o-PD electrodes displayed excellent linearity (R2 ≥ 0.997). In contrast, Ni-TMPP electrodes (with Nafion) had significantly poorer detection limits (76 ± 12 nM) and were less selective against dopamine (<5:1) and nitrite (<200:1). Ni-TMPP electrodes were also less linear than o-PD electrodes (R2 ≥ 0.911). Finally, we tested the in vitro and in vivo performance of the o-PD electrode in terms of its ability to detect NO. release from isolated rat renal arterioles and to measure NO. diffusion in the extracellular space of the rat brain.
AB - Nitric oxide (NO.) sensors were prepared using o-phenylenediamine (o-PD) and Nafion to modify the surface of 30 μm diameter carbon fiber electrodes. These electrodes were compared with nickel porphyrin-type NO. sensors that have already been described. High-speed chronoamperometry, amperometry, and differential pulse voltammetty were used to compare the performance of sensors modified with various combinations of Nafion, o-PD, or nickel(II) meso-tetrakis(3-methoxy-4-hydroxyphenyl)porphyrin (Ni-TMPP), in order to determine which electrodes had the most sensitivity and selectivity for NO.. Our findings showed that electrodes treated with Nafion first, followed by o-PD, were very sensitive to NO., with a detection limit of 35 ± 7 nM. In addition, o-PD electrodes were also very selective against ascorbate (>600:1), dopamine (>300:1), and nitrite (>900:1). Moreover, in the range of 0-6 μM NO., o-PD electrodes displayed excellent linearity (R2 ≥ 0.997). In contrast, Ni-TMPP electrodes (with Nafion) had significantly poorer detection limits (76 ± 12 nM) and were less selective against dopamine (<5:1) and nitrite (<200:1). Ni-TMPP electrodes were also less linear than o-PD electrodes (R2 ≥ 0.911). Finally, we tested the in vitro and in vivo performance of the o-PD electrode in terms of its ability to detect NO. release from isolated rat renal arterioles and to measure NO. diffusion in the extracellular space of the rat brain.
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U2 - 10.1021/ac960093w
DO - 10.1021/ac960093w
M3 - Article
C2 - 8694261
AN - SCOPUS:0030174099
SN - 0003-2700
VL - 68
SP - 2621
EP - 2628
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 15
ER -