Olfactory receptor surface expression is driven by association with the β2-adrenergic receptor

Chris Hague, Michelle A. Uberti, Zhongjian Chen, Cristina F. Bush, Seth V. Jones, Kerry J. Ressler, Randy A. Hall, Kenneth P. Minneman

Research output: Contribution to journalArticlepeer-review

105 Scopus citations


Olfactory receptors (ORs) comprise more than half of the large class I G protein-coupled receptor (GPCR) super-family. Although cloned over a decade ago, little is known about their properties because wild-type ORs do not efficiently reach the cell surface following heterologous expression. Receptor-receptor interactions strongly influence surface trafficking of other GPCRs, and we examined whether a similar mechanism might be involved in OR surface expression. Olfactory neurons are known to express β-adrenergic receptors (ARs), and we found that coexpression with β2-ARs, but not any other AR subtypes, dramatically increased mouse 71 (M71) OR surface expression in human embryonic kidney 293 cells. A persistent physical interaction between M71 ORs and β2-ARs was shown by coimmunoprecipitation and by cointernalization of the two receptors in response to their specific ligands. Also, coexpression of wild-type M71 ORs with β2-ARs resulted in cAMP responses to the M71 ligand acetophenone. Finally, in situ hybridization studies showed extensive colocalization of M71 OR and β2-AR expression in mouse olfactory epithelium. These data demonstrate the successful heterologous surface expression of a functional wild-type OR and reveal that persistent physical association with other GPCRs can control OR surface expression.

Original languageEnglish
Pages (from-to)13672-13676
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number37
StatePublished - Sep 14 2004

ASJC Scopus subject areas

  • General


Dive into the research topics of 'Olfactory receptor surface expression is driven by association with the β2-adrenergic receptor'. Together they form a unique fingerprint.

Cite this