On the Formation of Oxygenated Radicals by Fredericamycin A and Implications to Its Anticancer Activity: An ESR Investigation

N. S. Dalal; Xianglin Shi

doi:10.1021/bi00428a050

On the Formation of Oxygenated Radicals by Fredericamycin A and Implications to Its Anticancer Activity: An ESR Investigation

N. S. Dalal, Xianglin Shi

Toxicology and Cancer Biology

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Abstract

It has been recently suggested that the exceptionally high antitumor and antibacterial activity of natural fredericamycin A (FMA) is related to its ability to spontaneously generate the superoxide anion (O₂^-) and hydroxyl (^•OH) radicals in aerobic solutions [Hilton, B.D., Misra, R., & Zweier, J.L. (1986) Biochemistry 25, 5533]. With a view to understand the mechanistic details, attempts were made to reproduce earlier electron spin resonance (ESR) evidence for the oxygenated free radical formation in well-aerated solutions of natural FMA in dimethyl sulfoxide and dilute H₂O₂. Little or no evidence was obtained for the formation of the O₂^- and methoxy (•OCH₃) radicals, while the detected formation of the ^•OH and methyl (^•CH₃) radicals was attributable largely to mechanisms not involving FMA. These results thus reopen the question regarding the mechanism of its exceptionally high tumoricidal-bacteriocidal activity.

Original language	English
Pages (from-to)	748-750
Number of pages	3
Journal	Biochemistry
Volume	28
Issue number	2
DOIs	https://doi.org/10.1021/bi00428a050
State	Published - 1989

ASJC Scopus subject areas

Biochemistry

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title = "On the Formation of Oxygenated Radicals by Fredericamycin A and Implications to Its Anticancer Activity: An ESR Investigation",

abstract = "It has been recently suggested that the exceptionally high antitumor and antibacterial activity of natural fredericamycin A (FMA) is related to its ability to spontaneously generate the superoxide anion (O2-) and hydroxyl (•OH) radicals in aerobic solutions [Hilton, B.D., Misra, R., & Zweier, J.L. (1986) Biochemistry 25, 5533]. With a view to understand the mechanistic details, attempts were made to reproduce earlier electron spin resonance (ESR) evidence for the oxygenated free radical formation in well-aerated solutions of natural FMA in dimethyl sulfoxide and dilute H2O2. Little or no evidence was obtained for the formation of the O2- and methoxy (•OCH3) radicals, while the detected formation of the •OH and methyl (•CH3) radicals was attributable largely to mechanisms not involving FMA. These results thus reopen the question regarding the mechanism of its exceptionally high tumoricidal-bacteriocidal activity.",

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T1 - On the Formation of Oxygenated Radicals by Fredericamycin A and Implications to Its Anticancer Activity

T2 - An ESR Investigation

AU - Dalal, N. S.

AU - Shi, Xianglin

PY - 1989

Y1 - 1989

N2 - It has been recently suggested that the exceptionally high antitumor and antibacterial activity of natural fredericamycin A (FMA) is related to its ability to spontaneously generate the superoxide anion (O2-) and hydroxyl (•OH) radicals in aerobic solutions [Hilton, B.D., Misra, R., & Zweier, J.L. (1986) Biochemistry 25, 5533]. With a view to understand the mechanistic details, attempts were made to reproduce earlier electron spin resonance (ESR) evidence for the oxygenated free radical formation in well-aerated solutions of natural FMA in dimethyl sulfoxide and dilute H2O2. Little or no evidence was obtained for the formation of the O2- and methoxy (•OCH3) radicals, while the detected formation of the •OH and methyl (•CH3) radicals was attributable largely to mechanisms not involving FMA. These results thus reopen the question regarding the mechanism of its exceptionally high tumoricidal-bacteriocidal activity.

AB - It has been recently suggested that the exceptionally high antitumor and antibacterial activity of natural fredericamycin A (FMA) is related to its ability to spontaneously generate the superoxide anion (O2-) and hydroxyl (•OH) radicals in aerobic solutions [Hilton, B.D., Misra, R., & Zweier, J.L. (1986) Biochemistry 25, 5533]. With a view to understand the mechanistic details, attempts were made to reproduce earlier electron spin resonance (ESR) evidence for the oxygenated free radical formation in well-aerated solutions of natural FMA in dimethyl sulfoxide and dilute H2O2. Little or no evidence was obtained for the formation of the O2- and methoxy (•OCH3) radicals, while the detected formation of the •OH and methyl (•CH3) radicals was attributable largely to mechanisms not involving FMA. These results thus reopen the question regarding the mechanism of its exceptionally high tumoricidal-bacteriocidal activity.

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On the Formation of Oxygenated Radicals by Fredericamycin A and Implications to Its Anticancer Activity: An ESR Investigation

Abstract

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