Abstract
ESR spectra and scanning electron micrographs of human erythrocytes spin labeled with the conventional stearic acid nitroxide substituted at the 5-position have been obtained over a range of label-to-lipid ratios. While morphological changes as previously reported (Bieri V.G.; Wallach D.F.H.; Lin P.S. (1974) Proc. Natl. Acad. Sci. U. S. 71, 4797-4801) are reproduced, it is shown that at label-to-lipid ratios of 1 : 10 or less the basic ESR spectrum is not significantly affected. At low label concentrations the spin labeling technique is a viable one and can be used to investigate membrane properties.
Original language | English |
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Pages (from-to) | 697-702 |
Number of pages | 6 |
Journal | BBA - Biomembranes |
Volume | 426 |
Issue number | 4 |
DOIs | |
State | Published - Apr 5 1976 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Cell Biology