On the use of the spin labeling technique in the study of erythrocyte membranes

D. A. Butterfield, C. C. Whisnant, D. B. Chesnut

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

ESR spectra and scanning electron micrographs of human erythrocytes spin labeled with the conventional stearic acid nitroxide substituted at the 5-position have been obtained over a range of label-to-lipid ratios. While morphological changes as previously reported (Bieri V.G.; Wallach D.F.H.; Lin P.S. (1974) Proc. Natl. Acad. Sci. U. S. 71, 4797-4801) are reproduced, it is shown that at label-to-lipid ratios of 1 : 10 or less the basic ESR spectrum is not significantly affected. At low label concentrations the spin labeling technique is a viable one and can be used to investigate membrane properties.

Original languageEnglish
Pages (from-to)697-702
Number of pages6
JournalBBA - Biomembranes
Volume426
Issue number4
DOIs
StatePublished - Apr 5 1976

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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