Early acting cyclases play critical roles in programming the polyketide biosynthesis toward certain, distinguished scaffolds. Starting from acetyl-CoA and malonyl-CoA, a one-pot enzymatic total synthesis of an anthracyclinone scaffold, presteffimycinone, was achieved by mixing polyketide synthase (PKS) and early post-PKS enzymes from the biosynthetic pathways of three different types of type II-PKS driven anticancer antibiotics, namely, the mithramycin (aureolic acid-type), gilvocarcin (rearranged angucycline-type), and steffimycin (anthracycline) pathways.
|Number of pages||4|
|State||Published - Feb 3 2017|
Bibliographical noteFunding Information:
This work was supported by NIH grants CA 091901 and GM 105977 to J.R., and the Start-Up package provided by Florida Atlantic University Harbor Branch Oceanographic Institute Foundation to G.W. We thank Dr. Yi Tang, UCLA, for providing us the construct for MatB-expression.
© 2017 American Chemical Society.
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Organic Chemistry