@inproceedings{718a85491dbf446d995386b2b2cf1831,
title = "One-step isolation of transitory protein complexes with ifast",
abstract = "The ability to identify interactions between proteins is critical to understanding cellular processes. However, many of the complexes are transitory in nature, with half-lives of seconds or less. While important, these complexes are difficult to isolate as washing-based co-immunoprecipitation often results in inadvertent dissociation. Here, we present a technology for isolating complexes in a nearly instantaneous manner. Complexes are bound to paramagnetic particles (PMPs) and drawn through a barrier of immiscible liquid, thus purifying the complex from the bulk sample in a single step. Using model system, we have demonstrated the benefit of this method over traditional protocols.",
keywords = "Immunoprecipitation, Isolation, Microfluidics, Multi-phase, Protein complexes",
author = "Scott Berry and Lindsay Strotman and Emily Chin and Shawn Jackson and Nancy Thompson and Shigeki Miyamoto and Caroline Alexander and Richard Burgess and Beebe, {David J.}",
year = "2012",
language = "English",
isbn = "9780979806452",
series = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",
pages = "163--165",
booktitle = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",
note = "16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 ; Conference date: 28-10-2012 Through 01-11-2012",
}