Only minor differences in renal osteodystrophy features between wild-type and sclerostin knockout mice with chronic kidney disease

Daniel Cejka, Diego Parada-Rodriguez, Stefanie Pichler, Rodrig Marculescu, Ina Kramer, Michaela Kneissel, Thomas Gross, Andreas Reisinger, Dieter Pahr, Marie Claude Monier-Faugere, Martin Haas, Hartmut H. Malluche

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Renal osteodystrophy affects the majority of patients with advanced chronic kidney disease (CKD) and is characterized by progressive bone loss. This study evaluated the effects of sclerostin knockout on bone in a murine model of severe, surgically induced CKD in both sclerostin knockout and wild-type mice. Mice of both genotypes with normal kidney function served as controls. Tibiae were analyzed using micro-computed tomography, and lumbar vertebrae were analyzed by histomorphometry. Results were tested for statistical significance by 2-way ANOVA to investigate whether bone of the knockout mice reacted differently to CKD compared with bone of wild-type mice. In the tibiae, there was no difference after creation of CKD between wild-type and knockout animals for cortical thickness or cross-sectional moment of inertia. Increases in cortical porosity induced by CKD differed significantly between genotypes in the tibial metaphysis but not in the diaphysis. In the trabecular compartment, no difference in reaction to CKD between genotypes was found for bone volume, trabecular number, trabecular thickness, and trabecular separation. In the lumbar vertebrae, significant differences in response to CKD between wild-type and knockout mice were seen for both bone volume and trabecular thickness. Osteoblast parameters did not differ significantly, whereas osteoclast numbers significantly increased in the wild-type but significantly decreased in knockout mice with CKD. No differences in response to CKD between genotypes were found for bone formation rate or mineral apposition rate. Thus, complete absence of sclerostin has only minor effects on CKD-induced bone loss in mice.

Original languageEnglish
Pages (from-to)828-834
Number of pages7
JournalKidney International
Volume90
Issue number4
DOIs
StatePublished - Oct 1 2016

Bibliographical note

Funding Information:
We thank Dr. Raymonde Gagnon, McGill University Quebec, Canada, for assistance with introduction of the subtotal nephrectomy model at our laboratory and the staff of the animal research facility of the Medical University Vienna for technical assistance. This study was funded, in part, by the Kentucky Nephrology Research Trust.

Publisher Copyright:
© 2016 International Society of Nephrology

Keywords

  • bone
  • kidney disease
  • mouse
  • renal osteodystrophy
  • sclerostin

ASJC Scopus subject areas

  • Nephrology

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