Optically modulated fluorophores for selective fluorescence signal recovery

Chris I. Richards; Jung Cheng Hsiang; Dulal Senapati; Sandeep Patel; Junhua Yu; Tom Vosch; Robert M. Dickson

doi:10.1021/ja809785s

Optically modulated fluorophores for selective fluorescence signal recovery

Chris I. Richards, Jung Cheng Hsiang, Dulal Senapati, Sandeep Patel, Junhua Yu, Tom Vosch, Robert M. Dickson

Chemistry

Research output: Contribution to journal › Article › peer-review

121 Scopus citations

Abstract

Fluorescence imaging in biological sciences is hindered by significant depth-dependent signal attenuation and highly fluorescent backgrounds. We have developed optically modulated near-IR-emitting few-atom Ag nanodots that are selectively and dynamically photobrightened upon simultaneous excitation with a secondary laser, enabling high-sensitivity image extraction to reveal only the demodulated fluorophores. Image demodulation is demonstrated in high-background environments to extract weak signals from completely obscuring background emission.

Original language	English
Pages (from-to)	4619-4621
Number of pages	3
Journal	Journal of the American Chemical Society
Volume	131
Issue number	13
DOIs	https://doi.org/10.1021/ja809785s
State	Published - Apr 8 2009

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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abstract = "Fluorescence imaging in biological sciences is hindered by significant depth-dependent signal attenuation and highly fluorescent backgrounds. We have developed optically modulated near-IR-emitting few-atom Ag nanodots that are selectively and dynamically photobrightened upon simultaneous excitation with a secondary laser, enabling high-sensitivity image extraction to reveal only the demodulated fluorophores. Image demodulation is demonstrated in high-background environments to extract weak signals from completely obscuring background emission.",

author = "Richards, {Chris I.} and Hsiang, {Jung Cheng} and Dulal Senapati and Sandeep Patel and Junhua Yu and Tom Vosch and Dickson, {Robert M.}",

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T1 - Optically modulated fluorophores for selective fluorescence signal recovery

AU - Richards, Chris I.

AU - Hsiang, Jung Cheng

AU - Senapati, Dulal

AU - Patel, Sandeep

AU - Yu, Junhua

AU - Vosch, Tom

AU - Dickson, Robert M.

PY - 2009/4/8

Y1 - 2009/4/8

N2 - Fluorescence imaging in biological sciences is hindered by significant depth-dependent signal attenuation and highly fluorescent backgrounds. We have developed optically modulated near-IR-emitting few-atom Ag nanodots that are selectively and dynamically photobrightened upon simultaneous excitation with a secondary laser, enabling high-sensitivity image extraction to reveal only the demodulated fluorophores. Image demodulation is demonstrated in high-background environments to extract weak signals from completely obscuring background emission.

AB - Fluorescence imaging in biological sciences is hindered by significant depth-dependent signal attenuation and highly fluorescent backgrounds. We have developed optically modulated near-IR-emitting few-atom Ag nanodots that are selectively and dynamically photobrightened upon simultaneous excitation with a secondary laser, enabling high-sensitivity image extraction to reveal only the demodulated fluorophores. Image demodulation is demonstrated in high-background environments to extract weak signals from completely obscuring background emission.

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JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

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Optically modulated fluorophores for selective fluorescence signal recovery

Abstract

ASJC Scopus subject areas

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