Optimization of genome editing through CRISPR-Cas9 engineering

Jian Hua Zhang, Poorni Adikaram, Mritunjay Pandey, Allison Genis, William F. Simonds

Research output: Contribution to journalComment/debate

67 Scopus citations

Abstract

ABSTRACT: CRISPR (Clustered Regularly-Interspaced Short Palindromic Repeats)-Cas9 (CRISPR associated protein 9) has rapidly become the most promising genome editing tool with great potential to revolutionize medicine. Through guidance of a 20 nucleotide RNA (gRNA), CRISPR-Cas9 finds and cuts target protospacer DNA precisely 3 base pairs upstream of a PAM (Protospacer Adjacent Motif). The broken DNA ends are repaired by either NHEJ (Non-Homologous End Joining) resulting in small indels, or by HDR (Homology Directed Repair) for precise gene or nucleotide replacement. Theoretically, CRISPR-Cas9 could be used to modify any genomic sequences, thereby providing a simple, easy, and cost effective means of genome wide gene editing. However, the off-target activity of CRISPR-Cas9 that cuts DNA sites with imperfect matches with gRNA have been of significant concern because clinical applications require 100% accuracy. Additionally, CRISPR-Cas9 has unpredictable efficiency among different DNA target sites and the PAM requirements greatly restrict its genome editing frequency. A large number of efforts have been made to address these impeding issues, but much more is needed to fully realize the medical potential of CRISPR-Cas9. In this article, we summarize the existing problems and current advances of the CRISPR-Cas9 technology and provide perspectives for the ultimate perfection of Cas9-mediated genome editing.

Original languageEnglish
Pages (from-to)166-174
Number of pages9
JournalBioengineered
Volume7
Issue number3
DOIs
StatePublished - Apr 1 2016

Bibliographical note

Publisher Copyright:
© , This article is not subject to US copyright law.

Keywords

  • CRISPR-Cas9
  • efficiency
  • genome editing
  • specificity

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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