Optimized cell transplantation using adult rag2 mutant zebrafsh

Qin Tang; Nouran S. Abdelfattah; Jessica S. Blackburn; John C. Moore; Sarah A. Martinez; Finola E. Moore; Riadh Lobbardi; Inês M. Tenente; Myron S. Ignatius; Jason N. Berman; Robert S. Liwski; Yariv Houvras; David M. Langenau

doi:10.1038/nmeth.3031

Optimized cell transplantation using adult rag2 mutant zebrafsh

Qin Tang
, Nouran S. Abdelfattah
, Jessica S. Blackburn
, John C. Moore
, Sarah A. Martinez
, Finola E. Moore
, Riadh Lobbardi
, Inês M. Tenente
, Myron S. Ignatius
, Jason N. Berman
, Robert S. Liwski
, Yariv Houvras
, David M. Langenau

Molecular and Cellular Biochemistry

Research output: Contribution to journal › Article › peer-review

122 Scopus citations

Abstract

Cell transplantation into adult zebrafsh has lagged behind mouse models owing to the lack of immunocompromised strains. here we have created rag2^E450fs mutant zebrafsh that have reduced numbers of functional t and b cells but are viable and fecund. mutant fsh engraft muscle, blood stem cells and various cancers. rag2^E450fs mutant zebrafsh are the frst immunocompromised zebrafsh model that permits robust, long-term engraftment of multiple tissues and cancer.

Original language	English
Pages (from-to)	821-824
Number of pages	4
Journal	Nature Methods
Volume	11
Issue number	8
DOIs	https://doi.org/10.1038/nmeth.3031
State	Published - Aug 2014

Funding

Funders	Funder number
American Cancer Society-Michigan Cancer Research Fund
Leukemia and Lymphoma Society
National Institute of Diabetes and Digestive and Kidney Diseases	F32DK098875
National Institute of Diabetes and Digestive and Kidney Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth.3031

Cite this

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title = "Optimized cell transplantation using adult rag2 mutant zebrafsh",

abstract = "Cell transplantation into adult zebrafsh has lagged behind mouse models owing to the lack of immunocompromised strains. here we have created rag2E450fs mutant zebrafsh that have reduced numbers of functional t and b cells but are viable and fecund. mutant fsh engraft muscle, blood stem cells and various cancers. rag2E450fs mutant zebrafsh are the frst immunocompromised zebrafsh model that permits robust, long-term engraftment of multiple tissues and cancer.",

author = "Qin Tang and Abdelfattah, \{Nouran S.\} and Blackburn, \{Jessica S.\} and Moore, \{John C.\} and Martinez, \{Sarah A.\} and Moore, \{Finola E.\} and Riadh Lobbardi and Tenente, \{In{\^e}s M.\} and Ignatius, \{Myron S.\} and Berman, \{Jason N.\} and Liwski, \{Robert S.\} and Yariv Houvras and Langenau, \{David M.\}",

year = "2014",

month = aug,

doi = "10.1038/nmeth.3031",

language = "English",

volume = "11",

pages = "821--824",

number = "8",

}

TY - JOUR

T1 - Optimized cell transplantation using adult rag2 mutant zebrafsh

AU - Tang, Qin

AU - Abdelfattah, Nouran S.

AU - Blackburn, Jessica S.

AU - Moore, John C.

AU - Martinez, Sarah A.

AU - Moore, Finola E.

AU - Lobbardi, Riadh

AU - Tenente, Inês M.

AU - Ignatius, Myron S.

AU - Berman, Jason N.

AU - Liwski, Robert S.

AU - Houvras, Yariv

AU - Langenau, David M.

PY - 2014/8

Y1 - 2014/8

N2 - Cell transplantation into adult zebrafsh has lagged behind mouse models owing to the lack of immunocompromised strains. here we have created rag2E450fs mutant zebrafsh that have reduced numbers of functional t and b cells but are viable and fecund. mutant fsh engraft muscle, blood stem cells and various cancers. rag2E450fs mutant zebrafsh are the frst immunocompromised zebrafsh model that permits robust, long-term engraftment of multiple tissues and cancer.

AB - Cell transplantation into adult zebrafsh has lagged behind mouse models owing to the lack of immunocompromised strains. here we have created rag2E450fs mutant zebrafsh that have reduced numbers of functional t and b cells but are viable and fecund. mutant fsh engraft muscle, blood stem cells and various cancers. rag2E450fs mutant zebrafsh are the frst immunocompromised zebrafsh model that permits robust, long-term engraftment of multiple tissues and cancer.

UR - https://www.scopus.com/pages/publications/84905404249

UR - https://www.scopus.com/pages/publications/84905404249#tab=citedBy

U2 - 10.1038/nmeth.3031

DO - 10.1038/nmeth.3031

M3 - Article

C2 - 25042784

AN - SCOPUS:84905404249

SN - 1548-7091

VL - 11

SP - 821

EP - 824

JO - Nature Methods

JF - Nature Methods

IS - 8

ER -

Optimized cell transplantation using adult rag2 mutant zebrafsh

Abstract

Funding

UN SDGs

ASJC Scopus subject areas

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