Oral RNAi assays in Henosepilachna vigintioctopunctata suggest HvSec23 and HvSar1 as promising molecular targets for pest control

Mujuan Guo; Satyabrata Nanda; Chunxiao Yang; Zhaoyang Li; Junna Liu; Ran Gao; Youjun Zhang; Xuguo Zhou; Huipeng Pan

doi:10.1127/entomologia/2023/1712

Oral RNAi assays in Henosepilachna vigintioctopunctata suggest HvSec23 and HvSar1 as promising molecular targets for pest control

Mujuan Guo, Satyabrata Nanda, Chunxiao Yang, Zhaoyang Li, Junna Liu, Ran Gao, Youjun Zhang, Xuguo Zhou, Huipeng Pan

Entomology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The coat protein II (COPII) complex is a class of coated vesicles involved in protein transport from the endoplas-mic reticulum to the Golgi apparatus, serving a variety of important roles in animal growth and development. In this study, we selected three COPII genes, HvSec23, HvSec24, and HvSar1 to explore the RNAi silencing effect on Henosepilachna vigintioctopunctata (HVig), a dreadful solanaceous crop pest, using the dietary RNA interference (RNAi) technique. The results showed that HvSec23, HvSec24, and HvSar1 had a significant lethal effect on the 1^st and 3^rd instars of HVig. In addi-tion, oral administration of the bacterially expressed dsHvSec23 and dsHvSar1 caused high mortality and feeding inhibition in both larvae and adults, whereas dsHvSec24 only had a significant effect on the larvae. Moreover, the predatory ladybeetle Propylaea japonica was tested for evaluating the non-target effects of dsHvSec23 through oral and injection RNAi. Results confirmed that gene expression of PjSec23 was significantly suppressed by injection RNAi, but not oral RNAi. Overall, our results revealed that HvSec23 and HvSar1 could be used as promising molecular targets for controlling HVig.

Original language	English
Pages (from-to)	147-155
Number of pages	9
Journal	Entomologia Generalis
Volume	43
Issue number	1
DOIs	https://doi.org/10.1127/entomologia/2023/1712
State	Published - 2023

Bibliographical note

Funding Information:
Acknowledgments: This research was supported by the National Natural Science Foundation of China (32172500, 31972269). No role has been played by the aforementioned funding agencies in designing, executing, and publishing the study.

Publisher Copyright:
© 2022 E. Schweizerbart’sche Verlagsbuchhandlung, 70176 Stuttgart, Germany.

Keywords

Propylaea japonica
RNA interference
coat protein II complex
mortality
non-target effect
risk assessment

ASJC Scopus subject areas

Insect Science

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10.1127/entomologia/2023/1712

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@article{f877797fe4d3442fb10a8a8464729a45,

title = "Oral RNAi assays in Henosepilachna vigintioctopunctata suggest HvSec23 and HvSar1 as promising molecular targets for pest control",

abstract = "The coat protein II (COPII) complex is a class of coated vesicles involved in protein transport from the endoplas-mic reticulum to the Golgi apparatus, serving a variety of important roles in animal growth and development. In this study, we selected three COPII genes, HvSec23, HvSec24, and HvSar1 to explore the RNAi silencing effect on Henosepilachna vigintioctopunctata (HVig), a dreadful solanaceous crop pest, using the dietary RNA interference (RNAi) technique. The results showed that HvSec23, HvSec24, and HvSar1 had a significant lethal effect on the 1st and 3rd instars of HVig. In addi-tion, oral administration of the bacterially expressed dsHvSec23 and dsHvSar1 caused high mortality and feeding inhibition in both larvae and adults, whereas dsHvSec24 only had a significant effect on the larvae. Moreover, the predatory ladybeetle Propylaea japonica was tested for evaluating the non-target effects of dsHvSec23 through oral and injection RNAi. Results confirmed that gene expression of PjSec23 was significantly suppressed by injection RNAi, but not oral RNAi. Overall, our results revealed that HvSec23 and HvSar1 could be used as promising molecular targets for controlling HVig.",

keywords = "Propylaea japonica, RNA interference, coat protein II complex, mortality, non-target effect, risk assessment",

author = "Mujuan Guo and Satyabrata Nanda and Chunxiao Yang and Zhaoyang Li and Junna Liu and Ran Gao and Youjun Zhang and Xuguo Zhou and Huipeng Pan",

note = "Funding Information: Acknowledgments: This research was supported by the National Natural Science Foundation of China (32172500, 31972269). No role has been played by the aforementioned funding agencies in designing, executing, and publishing the study. Publisher Copyright: {\textcopyright} 2022 E. Schweizerbart{\textquoteright}sche Verlagsbuchhandlung, 70176 Stuttgart, Germany.",

year = "2023",

doi = "10.1127/entomologia/2023/1712",

language = "English",

volume = "43",

pages = "147--155",

journal = "Entomologia Generalis",

issn = "0171-8177",

publisher = "E. Schweizerbart'sche Verlagsbuchhandlung",

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T1 - Oral RNAi assays in Henosepilachna vigintioctopunctata suggest HvSec23 and HvSar1 as promising molecular targets for pest control

AU - Guo, Mujuan

AU - Nanda, Satyabrata

AU - Yang, Chunxiao

AU - Li, Zhaoyang

AU - Liu, Junna

AU - Gao, Ran

AU - Zhang, Youjun

AU - Zhou, Xuguo

AU - Pan, Huipeng

N1 - Funding Information: Acknowledgments: This research was supported by the National Natural Science Foundation of China (32172500, 31972269). No role has been played by the aforementioned funding agencies in designing, executing, and publishing the study. Publisher Copyright: © 2022 E. Schweizerbart’sche Verlagsbuchhandlung, 70176 Stuttgart, Germany.

PY - 2023

Y1 - 2023

N2 - The coat protein II (COPII) complex is a class of coated vesicles involved in protein transport from the endoplas-mic reticulum to the Golgi apparatus, serving a variety of important roles in animal growth and development. In this study, we selected three COPII genes, HvSec23, HvSec24, and HvSar1 to explore the RNAi silencing effect on Henosepilachna vigintioctopunctata (HVig), a dreadful solanaceous crop pest, using the dietary RNA interference (RNAi) technique. The results showed that HvSec23, HvSec24, and HvSar1 had a significant lethal effect on the 1st and 3rd instars of HVig. In addi-tion, oral administration of the bacterially expressed dsHvSec23 and dsHvSar1 caused high mortality and feeding inhibition in both larvae and adults, whereas dsHvSec24 only had a significant effect on the larvae. Moreover, the predatory ladybeetle Propylaea japonica was tested for evaluating the non-target effects of dsHvSec23 through oral and injection RNAi. Results confirmed that gene expression of PjSec23 was significantly suppressed by injection RNAi, but not oral RNAi. Overall, our results revealed that HvSec23 and HvSar1 could be used as promising molecular targets for controlling HVig.

AB - The coat protein II (COPII) complex is a class of coated vesicles involved in protein transport from the endoplas-mic reticulum to the Golgi apparatus, serving a variety of important roles in animal growth and development. In this study, we selected three COPII genes, HvSec23, HvSec24, and HvSar1 to explore the RNAi silencing effect on Henosepilachna vigintioctopunctata (HVig), a dreadful solanaceous crop pest, using the dietary RNA interference (RNAi) technique. The results showed that HvSec23, HvSec24, and HvSar1 had a significant lethal effect on the 1st and 3rd instars of HVig. In addi-tion, oral administration of the bacterially expressed dsHvSec23 and dsHvSar1 caused high mortality and feeding inhibition in both larvae and adults, whereas dsHvSec24 only had a significant effect on the larvae. Moreover, the predatory ladybeetle Propylaea japonica was tested for evaluating the non-target effects of dsHvSec23 through oral and injection RNAi. Results confirmed that gene expression of PjSec23 was significantly suppressed by injection RNAi, but not oral RNAi. Overall, our results revealed that HvSec23 and HvSar1 could be used as promising molecular targets for controlling HVig.

KW - Propylaea japonica

KW - RNA interference

KW - coat protein II complex

KW - mortality

KW - non-target effect

KW - risk assessment

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U2 - 10.1127/entomologia/2023/1712

DO - 10.1127/entomologia/2023/1712

M3 - Article

AN - SCOPUS:85153409629

SN - 0171-8177

VL - 43

SP - 147

EP - 155

JO - Entomologia Generalis

JF - Entomologia Generalis

IS - 1

ER -

Oral RNAi assays in Henosepilachna vigintioctopunctata suggest HvSec23 and HvSar1 as promising molecular targets for pest control

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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