Oral RNAi toxicity assay suggests clathrin heavy chain as a promising molecular target for controlling the 28-spotted potato ladybird, Henosepilachna vigintioctopunctata

Mujuan Guo, Satyabrata Nanda, Shimin Chen, Lü Jing, Chunxiao Yang, Zhuoqi Liu, Wei Guo, Baoli Qiu, Youjun Zhang, Xuguo Zhou, Huipeng Pan

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

BACKGROUND: Use of RNA interference (RNAi) technology in effective pest management has been explored for decades. Henosepilachna vigintioctopunctata is a major solanaceous crop pest in Asia. In this study, the effects of the RNAi-mediated silencing of clathrin heavy chain in H. vigintioctopunctata were investigated. RESULTS: Feeding either the in vitro-synthesized or the bacterially expressed double-stranded RNAs (dsRNAs) significantly impaired the normal physiology of H. vigintioctopunctata instars and adults. However, the bacterially expressed dsHvChc caused higher mortality than the in vitro-synthesized ones in the larvae and adults. Moreover, on evaluating the potential risk of dsHvChc on Propylea japonica, significant transcriptional effects of dsHvChc1 were observed, while the organismal level effects were not significant. On the contrary, dsHvChc2 did not affect P. japonica at either level. A similar test revealed significant transcriptional effects of dsPjChc1 on H. vigintioctopunctata, while staying ineffective at the organismal levels. Conversely, dsPjChc2 did not affect H. vigintioctopunctata at either level. Importantly, no effect of dsPjChc1 exposure on H. vigintioctopunctata suggested that other factors besides the 21-nucleotide (nt) matches between sequences were responsible. Finally, ingestion of dsHvmChc1 derived from H. vigintioctomaculata, containing 265-nt matches with dsHvChc1, caused 100% mortality in H. vigintioctopunctata. CONCLUSIONS: We conclude that (i) species with numerous 21-nt matches in homologous genes are more likely to be susceptible to dsRNA; (ii) dsRNA can be safely designed to avoid negative effects on non-target organisms at both transcriptional and organismal levels; (iii) HvChc can be used as an efficient RNAi target gene to effectively manage H. vigintioctopunctata.

Original languageEnglish
Pages (from-to)3871-3879
Number of pages9
JournalPest Management Science
Volume78
Issue number9
DOIs
StatePublished - Sep 2022

Bibliographical note

Publisher Copyright:
© 2021 Society of Chemical Industry.

Keywords

  • RNAi product
  • biopesticide
  • eco-friendly pest control
  • ladybird pest
  • non-target effect

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Insect Science

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