Orally delivered dsRNA induces knockdown of target genes and mortality in the Asian long-horned beetle, Anoplophora glabripennis

Ramesh Kumar Dhandapani, Jian Jun Duan, Subba Reddy Palli

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The Asian long-horned beetle (ALB) Anoplophora glabripennis is a serious invasive forest pest in several countries, including the United States. Methods available to manage or eradicate this pest are extremely limited, but RNA interference (RNAi) technology is a potentially effective method to control ALB. In this study, we used sucrose feeding bioassay for oral delivery of double-strand RNA (dsRNA) to ALB larvae. 32P-labeled dsRNA orally delivered to ALB larvae using the sucrose droplet feeding method was processed to small interfering RNA. Feeding neonate larvae with dsRNA targeting genes coding for the inhibitor of apoptosis (IAP), vacuolar sorting protein SNF7 (SNF7), and snakeskin (SSK) induced knockdown of target genes and mortality. Feeding 2 µg of dsRNA per day for 3 days did not induce a significant decrease in the expression of target genes or mortality. However, feeding 5 or 10 µg of dsRNA per day for 3 days induced a significant decrease in the expression of target genes and 50–90% mortality. Interestingly, feeding 2.5 µg each of dsIAP plus dsSNF7, dsIAP plus dsSSK, or dsSNF7 plus dsSSK per day for 3 days induced a significant decrease in the expression of both target genes and approximately 80% mortality. Our findings demonstrate that orally delivered dsRNA induces target gene knockdown and mortality in ALB neonate larvae and RNAi technology may have the potential for effective ALB control.

Original languageEnglish
Article numbere21679
JournalArchives of Insect Biochemistry and Physiology
Volume104
Issue number4
DOIs
StatePublished - Aug 1 2020

Bibliographical note

Publisher Copyright:
© 2020 Wiley Periodicals, Inc.

Keywords

  • ALB
  • dsIAP
  • dsSNF7
  • feeding RNAi
  • pest control

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Insect Science

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