Organisation and molecular analysis of repeated DNA sequences in the rice blast fungus Magnaporthe grisea

Pradeep Kachroo; Munish Ahuja; Sally A. Leong; Bharat B. Chattoo

doi:10.1007/s002940050217

Organisation and molecular analysis of repeated DNA sequences in the rice blast fungus Magnaporthe grisea

Pradeep Kachroo, Munish Ahuja, Sally A. Leong, Bharat B. Chattoo

Plant Pathology

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

The distribution of a previously described repeated DNA sequence present as a 1.3-kb PstI fragment in the genome of the rice blast fungus Magnaporthe grisea was analysed by carrying out DNA fingerprint analysis of 36 isolates including rice, non-rice and laboratory strains. The analysis of various higher-molecular-weight PstI fragments with homology to the 1.3-kb repeat revealed that these may arise predominantly from transposon insertions or point mutations. Analysis of a 5.1-kb derivative revealed both a point mutation at a PstI site and an insertion of a putative transposable element which caused an increase in molecular weight from 1.3 to 5.1 kb. Another repeat element of 1.4 kb was identified and found to exist in association with the 1.3-kb repeat. Both 1.3- and 1.4-kb elements were found to be parts of MGR583, a LINE-like element. These elements were present in a high copy number in all the rice and a majority of non-rice pathogens indicating that MGR583 is not a host-specific sequence as reported earlier. Our results suggest that repeated DNA elements in M. grisea have amplified independently of one another and further indicate that different isolates of M. grisea may have evolved from several distinct lines of origin.

Original language	English
Pages (from-to)	361-369
Number of pages	9
Journal	Current Genetics
Volume	31
Issue number	4
DOIs	https://doi.org/10.1007/s002940050217
State	Published - Apr 1997

Bibliographical note

Funding Information:
Acknowledgements We thank Drs. Naoto Nitta, (Department of Plant Pathology, University of Wisconsin, Madison, USA) for providing the DNA samples of progeny obtained from a cross between Guy11 and 2539, and Mark Farman (Department of Plant Pathology, University of Wisconsin, Madison, USA) for providing the DNA samples of some of the non-rice pathogens, for the Pot3 clone and also for sharing his unpublished data. Finally, we thank Dr. Autar Mattoo (USDA, Beltsville, M.D., USA) for his critical reading of the manuscript. This work was supported by grants from the Department of Biotechnology, Government of India, and by the Rockefeller Foundation to B.B.C., as well as by the United States Department of Agriculture and the Rockefeller Foundation to S.A.L.

Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.

Keywords

Fingerprinting
Repeated DNA
Rice blast
Transposon

ASJC Scopus subject areas

Genetics

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10.1007/s002940050217

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@article{7774deaaa198445681e1dabc15d08c45,

title = "Organisation and molecular analysis of repeated DNA sequences in the rice blast fungus Magnaporthe grisea",

abstract = "The distribution of a previously described repeated DNA sequence present as a 1.3-kb PstI fragment in the genome of the rice blast fungus Magnaporthe grisea was analysed by carrying out DNA fingerprint analysis of 36 isolates including rice, non-rice and laboratory strains. The analysis of various higher-molecular-weight PstI fragments with homology to the 1.3-kb repeat revealed that these may arise predominantly from transposon insertions or point mutations. Analysis of a 5.1-kb derivative revealed both a point mutation at a PstI site and an insertion of a putative transposable element which caused an increase in molecular weight from 1.3 to 5.1 kb. Another repeat element of 1.4 kb was identified and found to exist in association with the 1.3-kb repeat. Both 1.3- and 1.4-kb elements were found to be parts of MGR583, a LINE-like element. These elements were present in a high copy number in all the rice and a majority of non-rice pathogens indicating that MGR583 is not a host-specific sequence as reported earlier. Our results suggest that repeated DNA elements in M. grisea have amplified independently of one another and further indicate that different isolates of M. grisea may have evolved from several distinct lines of origin.",

keywords = "Fingerprinting, Repeated DNA, Rice blast, Transposon",

author = "Pradeep Kachroo and Munish Ahuja and Leong, {Sally A.} and Chattoo, {Bharat B.}",

note = "Funding Information: Acknowledgements We thank Drs. Naoto Nitta, (Department of Plant Pathology, University of Wisconsin, Madison, USA) for providing the DNA samples of progeny obtained from a cross between Guy11 and 2539, and Mark Farman (Department of Plant Pathology, University of Wisconsin, Madison, USA) for providing the DNA samples of some of the non-rice pathogens, for the Pot3 clone and also for sharing his unpublished data. Finally, we thank Dr. Autar Mattoo (USDA, Beltsville, M.D., USA) for his critical reading of the manuscript. This work was supported by grants from the Department of Biotechnology, Government of India, and by the Rockefeller Foundation to B.B.C., as well as by the United States Department of Agriculture and the Rockefeller Foundation to S.A.L. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",

year = "1997",

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doi = "10.1007/s002940050217",

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N2 - The distribution of a previously described repeated DNA sequence present as a 1.3-kb PstI fragment in the genome of the rice blast fungus Magnaporthe grisea was analysed by carrying out DNA fingerprint analysis of 36 isolates including rice, non-rice and laboratory strains. The analysis of various higher-molecular-weight PstI fragments with homology to the 1.3-kb repeat revealed that these may arise predominantly from transposon insertions or point mutations. Analysis of a 5.1-kb derivative revealed both a point mutation at a PstI site and an insertion of a putative transposable element which caused an increase in molecular weight from 1.3 to 5.1 kb. Another repeat element of 1.4 kb was identified and found to exist in association with the 1.3-kb repeat. Both 1.3- and 1.4-kb elements were found to be parts of MGR583, a LINE-like element. These elements were present in a high copy number in all the rice and a majority of non-rice pathogens indicating that MGR583 is not a host-specific sequence as reported earlier. Our results suggest that repeated DNA elements in M. grisea have amplified independently of one another and further indicate that different isolates of M. grisea may have evolved from several distinct lines of origin.

AB - The distribution of a previously described repeated DNA sequence present as a 1.3-kb PstI fragment in the genome of the rice blast fungus Magnaporthe grisea was analysed by carrying out DNA fingerprint analysis of 36 isolates including rice, non-rice and laboratory strains. The analysis of various higher-molecular-weight PstI fragments with homology to the 1.3-kb repeat revealed that these may arise predominantly from transposon insertions or point mutations. Analysis of a 5.1-kb derivative revealed both a point mutation at a PstI site and an insertion of a putative transposable element which caused an increase in molecular weight from 1.3 to 5.1 kb. Another repeat element of 1.4 kb was identified and found to exist in association with the 1.3-kb repeat. Both 1.3- and 1.4-kb elements were found to be parts of MGR583, a LINE-like element. These elements were present in a high copy number in all the rice and a majority of non-rice pathogens indicating that MGR583 is not a host-specific sequence as reported earlier. Our results suggest that repeated DNA elements in M. grisea have amplified independently of one another and further indicate that different isolates of M. grisea may have evolved from several distinct lines of origin.

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Organisation and molecular analysis of repeated DNA sequences in the rice blast fungus Magnaporthe grisea

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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