Organism dual RNA-seq reveals the importance of BarA/UvrY in Vibrio parahaemolyticus virulence

Wenwen Zhang, Ruiqiang Xie, Xiaohua Douglas Zhang, Leo Tsz On Lee, Hongjie Zhang, Menghua Yang, Bo Peng, Jun Zheng

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Elucidation of host-pathogen interaction is essential for developing effective strategies to combat bacterial infection. Dual RNA-Seq using cultured cells or tissues/organs as the host of pathogen has emerged as a novel strategy to understand the responses concurrently from both pathogen and host at cellular level. However, bacterial infection mostly causes systematic responses from the host at organism level where the interplay is urgently to be understood but inevitably being neglected by the current practice. Here, we developed an approach that simultaneously monitor the genome-wide infection-linked transcriptional alterations in both pathogenic Vibrio parahaemolyticus and the infection host nematode Caenorhabditis elegans. Besides the dynamic alterations in transcriptomes of both C. elegans and V. parahaemolyticus during infection, we identify a two-component system, BarA/UvrY, that is important for virulence in host. BarA/UvrY not only controls the virulence factors in V. parahaemolyticus including Type III and Type VI secretion systems, but also attenuates innate immune responses in C. elegans, including repression on the MAP kinase-mediated cascades. Thus, our study exemplifies the use of dual RNA-Seq at organism level to uncover previously unrecognized interplay between host and pathogen.

Original languageEnglish
Pages (from-to)7561-7577
Number of pages17
JournalFASEB Journal
Volume34
Issue number6
DOIs
StatePublished - Jun 1 2020

Bibliographical note

Publisher Copyright:
© 2020 Federation of American Societies for Experimental Biology

Keywords

  • BarA
  • C. elegans
  • UvrY
  • V. parahaemolyticus
  • organism dual RNA-Seq
  • virulence factor

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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